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Merck
CN

1.11608

Ethidium bromide

(1% solution in water) for electrophoresis

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About This Item

NACRES:
NA.51
UNSPSC Code:
41105305
Technical Service
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Quality Level

form

liquid

pH

4.4 (20 °C in H2O)

density

1.00 g/cm3 at 20 °C

storage temp.

2-8°C

Application

Ethidium bromide has been used to stain polyacrylamide gels for small-scale transcription reactions. It has also been used for the quantification of DNA.

Biochem/physiol Actions

Ethidium bromide is a red fluorescent dye that reacts with the DNA of membrane-damaged cells by intercalating. It is used for visualizing DNA in gels. Ethidium bromide is carcinogenic with moderate toxicity.

Features and Benefits

Robust and easy to use

Analysis Note

Identity (UV/VIS-Spectrum): passes test
Absorption maximum (water): 478 - 482 nm
Spec. Absorptivity A 1%/1cm (λmax; 0.004 %; water): 140 - 160
Suitability for electrophoresis: passes test


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pictograms

Skull and crossbonesHealth hazard

signalword

Danger

Hazard Classifications

Acute Tox. 3 Inhalation - Muta. 2

wgk

nwg

flash_point_f

Not applicable

flash_point_c

Not applicable

hcodes

Storage Class

6.1D - Non-combustible acute toxic Cat.3 / toxic hazardous materials or hazardous materials causing chronic effects



Certificates of Analysis (COA)

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Global Trade Item Number

SKUGTIN
111608003004022536133775