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1.12553

Coomassie Brilliant blue R 250 (C.I. 42660)

Name: Coomassie Brilliant blue R 250 (C.I. 42660)
Brand: MM

for electrophoresis Trademark of Imperial Chemical Industries PLC

Synonym(s):

Coomassie Brilliant blue R 250 (C.I. 42660), protein stain

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About This Item

Empirical Formula (Hill Notation):

C₄₅H₄₄N₃NaO₇S₂

CAS Number:

6104-59-2

Molecular Weight:

825.97

UNSPSC Code:

41105305

EC Index Number:

228-060-5

NACRES:

NC.01

MDL number:

MFCD00041762

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Properties

Quality Level

100

potency

>5000 mg/kg LD₅₀, oral (Rat)

technique(s)

protein staining: suitable

pH

6.2 (25 °C, 10 g/L in H₂O)

mp

174-180 °C

solubility

20 g/L

bulk density

250 kg/m³

ε_max

≥300 at 554-563 nm at 0.025 g/L (pH 7.0)

application(s)

diagnostic assay manufacturing
hematology
histology

storage temp.

2-30°C

SMILES string

[Na+].[S](=O)(=O)([O-])c1cc(ccc1)CN(CC)c2ccc(cc2)[C+](c5ccc(cc5)N(CC)Cc6cc(ccc6)[S](=O)(=O)[O-])c3ccc(cc3)Nc4ccc(cc4)OCC

InChI

1S/C45H45N3O7S2.Na/c1-4-47(31-33-9-7-11-43(29-33)56(49,50)51)40-23-15-36(16-24-40)45(35-13-19-38(20-14-35)46-39-21-27-42(28-22-39)55-6-3)37-17-25-41(26-18-37)48(5-2)32-34-10-8-12-44(30-34)57(52,53)54;/h7-30,46H,4-6,31-32H2,1-3H3,(H-,49,50,51,52,53,54);/q;+1/p-1

InChI key

HAEGGLCXLFWTBE-UHFFFAOYSA-M

Description

Analysis Note

Identity (UV/VIS-Spectrum): passes test
Absorption maximum λ_max. (buffer pH 7.0): 554 - 563 nm
Spec. Absorptivity A 1%/1cm (λ_max; 0.025 g/l; buffer pH 7.0; calc. on dried substance): ≥ 300
TLC-Test: passes test
Loss on drying (110 °C): ≤ 5 %
Suitability for electrophoresis: passes test

Legal Information

Storage Class

11 - Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c