1.15939
Nuclear fast red (C.I. 60760)
for microscopy Certistain®
Synonym(s):
Nuclear fast red (C.I. 60760)
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About This Item
Empirical Formula (Hill Notation):
C14H8NNaO7S
CAS Number:
Molecular Weight:
357.27
EC Index Number:
229-088-0
UNSPSC Code:
41116130
MDL number:
Quality Segment
form
solid
composition
Dye, ≥90% spectrophotometric assay
pH
5.5-7.0 (20 °C, 1 g/L in H2O)
solubility
1 g/L
bulk density
400‑450 kg/m3
εmax
380-422 at 557-559 nm in DMSO at 0.02 g/L, 383-426 at 520-522 nm in DMSO at 0.02 g/L
application(s)
diagnostic assay manufacturing
hematology
histology
storage temp.
2-30°C
InChI
1S/C14H9NO7S.Na/c15-9-7-8(12(18)14(13(9)19)23(20,21)22)11(17)6-4-2-1-3-5(6)10(7)16;/h1-4,18-19H,15H2,(H,20,21,22);/q;+1/p-1
InChI key
IFSXZLJQEKGQAF-UHFFFAOYSA-M
General description
Nuclear fast red (C.I. 60760) for microscopy Certistain®, is a dry dye for microscopy that is often used for staining nuclei in sample material of human origin. Depending on user requirements and personal preferences, it can be used for the preparation of a Nuclear fast red staining solution. It is commonly used for counterstaining in histochemical reactions, e.g. in Berlin blue staining for detection of iron or in silver plating of reticulin fibers, as well as nuclear staining with Alcian blue. As the dye belongs to the portfolio of Certistain® dyes, it is chemically analyzed according to strict specifications before release (CE marked and IVD product) and a high staining quality, with reliable staining results is guaranteed. For more details, please see instructions for use (IFU). The IFU can be downloaded from this webpage.
Analysis Note
Dye content (spectrophotometrically): ≥ 90 %
Identity
- sodium:
passes test
- UV/VIS-Spectrum: passes test
Absorption maximum λ 1 (Dimethyl sulfoxide): 557 - 559 nm
Absorption maximum λ 2 (Dimethyl sulfoxide): 520 - 522 nm
Spec. Absorptivity A 1%/1cm
- λ1max; 0.02 g/l; dimethyl sulphoxide:
380 - 422
- λ2max; 0.02 g/l; Dimethyl sulphoxide: 383 - 426
TLC-Test: passes test
Loss on drying (110 °C): ≤ 5 %
Suitability for microscopy: passes test
Identity
- sodium:
passes test
- UV/VIS-Spectrum: passes test
Absorption maximum λ 1 (Dimethyl sulfoxide): 557 - 559 nm
Absorption maximum λ 2 (Dimethyl sulfoxide): 520 - 522 nm
Spec. Absorptivity A 1%/1cm
- λ1max; 0.02 g/l; dimethyl sulphoxide:
380 - 422
- λ2max; 0.02 g/l; Dimethyl sulphoxide: 383 - 426
TLC-Test: passes test
Loss on drying (110 °C): ≤ 5 %
Suitability for microscopy: passes test
Legal Information
CERTISTAIN is a registered trademark of Merck KGaA, Darmstadt, Germany
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Storage Class
11 - Combustible Solids
WGK
WGK 3
Flash Point (°C)
Not applicable
Flash Point (°F)
Not applicable
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