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1.15943

Methylene blue (C.I.52015)

for microscopy Certistain®

Synonym(s):

Methylene Blue trihydrate, Methylthioninium chloride, 3,7-Bis(dimethylamino)phenothiazinium chloride, Tetramethylthionine chloride

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About This Item

Empirical Formula (Hill Notation):
C16H18ClN3S · 3H2O
CAS Number:
7220-79-3
Molecular Weight:
373.90
EC Index Number:
200-515-2
UNSPSC Code:
41116130
Beilstein/REAXYS Number:
3599847
MDL number:
MFCD00012111
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Quality Level

400

form

solid

potency

1180 mg/kg LD50, oral (Rat)

composition

Dye, ≥82% spectrophotometric assay

pH

3 (20 °C, 10 g/L in H2O)

mp

190 °C (dec.) (lit.)

solubility

50 g/L

density

1.757 g/cm3 at 22 °C

bulk density

400‑600 kg/m3

εmax

2250-2750 at 660-665 nm in 50% ethanol at 0.003 g/L

application(s)

diagnostic assay manufacturing
hematology
histology

storage temp.

2-30°C

SMILES string

[Cl-].CN(C)c1ccc2N=C3C=C\C(C=C3Sc2c1)=[N+](/C)C

InChI

1S/C16H18N3S.ClH/c1-18(2)11-5-7-13-15(9-11)20-16-10-12(19(3)4)6-8-14(16)17-13;/h5-10H,1-4H3;1H/q+1;/p-1

InChI key

CXKWCBBOMKCUKX-UHFFFAOYSA-M

General description

Methylene blue (C.I. 52015) for microscopy Certistain® is a dry dye that is used in bacteriology for an overview stain and as counterstaining for Ziehl-Neelsen method in sample material of human origin. Bacteria appear blue. The dye has a further important application in hematology: Methylene blue and eosin are the main components of many staining solutions and dye mixtures. Certistain® is the product group for seleceted high quality dyes that fulfill all quality requirements of IVD and CE registration. For more details, please see instructions for use (IFU). The IFU can be downloaded from this webpage.

Analysis Note

Dye content (spectrophotometrically): ≥ 82 %
Identity
- Chloride:
passes test
- UV/VIS-Spectrum: passes test
Absorption maximum λ max (ethanol 50 %): 660 - 665 nm
Spec. Absorptivity A 1%/1cm (λmax; 0.003 g/l; ethanol 50 %): 2250 - 2750
TLC-Test: passes test
Loss on drying (110 °C): 10 - 15 %
Suitability for microscopy: passes test

Legal Information

CERTISTAIN is a registered trademark of Merck KGaA, Darmstadt, Germany

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pictograms

Exclamation mark
GHS07

signalword

Warning

hcodes

H302

Hazard Classifications

Acute Tox. 4 Oral

Storage Class

11 - Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

Regulatory Information

监管及禁止进口产品

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Certificates of Analysis (COA)

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