12-301
Non-Stimulated A431 Cell Lysate
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About This Item
UNSPSC Code:
41105330
NACRES:
NA.41
eCl@ss:
32011807
Technique(s):
western blot: suitable
Species reactivity:
-
Application:
WB
Citations:
7
biological source
human
Quality Level
form
liquid
manufacturer/tradename
Upstate®
technique(s)
western blot: suitable
shipped in
dry ice
Biochem/physiol Actions
A431
Physical form
modified RIPA buffer (50mM Tris-HCl, pH 7.4, 1% NP40, 0.25% sodium deoxycholate, 150mM NaCl, 1mM EDTA, 1mM PMSF, 1μg/ml each of aprotinin, leupeptin, pepstatin, 1mM Na3VO4, 1mM NaF) and diluted with non-reducing sample buffer (31mM Tris-HCl, pH 6.8, 5% glycerol, 1% SDS, 0.002% bromphenol blue).
Preparation Note
1 year at -20°C
Analysis Note
Routinely evaluated as a positive control for several of Upstates antibodies.
Legal Information
UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage Class
12 - Non Combustible Liquids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
signalword
Danger
hcodes
Hazard Classifications
Aquatic Chronic 3 - ED ENV 1 - Eye Irrit. 2
Regulatory Information
高风险级别生物产品-Merck
This item has
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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D J Flint et al.
Journal of molecular endocrinology, 36(3), 435-448 (2006-05-25)
Insulin-like growth factor-binding protein 5 (IGFBP-5) mediates involution of the mammary gland. The decrease in DNA content and mammary gland weight which accompanies involution was inhibited by prolactin (PRL) in wild-type but not transgenic mice expressing IGFBP-5. Phospho-STAT5 protein levels
Norikazu Yabuta et al.
Scientific reports, 8(1), 4892-4892 (2018-03-22)
p63, a transcriptional factor that belongs to the p53 family, regulates epidermal differentiation, stemness, cell death, tumorigenesis, metastasis, and senescence. However, its molecular mechanism remains elusive. We report here that TAp63 phosphorylated at T46/T281 specifically upregulates the late cornified envelope
Yu Shi et al.
Molecular and cellular biology, 23(21), 7732-7741 (2003-10-16)
MK5 (mitogen-activated protein kinase [MAPK]-activated protein kinase 5), also designated PRAK (p38-regulated and -activated kinase), was deleted from mice by homologous recombination. Although no MK5 full-length protein and kinase activity was detected in the MK5 knockout mice, the animals were
Global Trade Item Number
| SKU | GTIN |
|---|---|
| 12-301 | 08436037123535 |
| 1009610001 | 04022536869223 |