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Merck
CN

12-303

Jurkat Cell Lysate

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About This Item

UNSPSC Code:
41105330
NACRES:
NA.41
eCl@ss:
32011807
Technique(s):
western blot: suitable
Species reactivity:
-
Application:
WB
Citations:
7
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biological source

human

form

liquid

manufacturer/tradename

Upstate®

technique(s)

western blot: suitable

shipped in

dry ice

Quality Level

Biochem/physiol Actions

Jurkat

Physical form

Modified RIPA buffer (50mM Tris-HCl, pH 7.4, 1% NP40, 0.25% sodium deoxycholate, 150mM NaCl, 1mM EDTA, 1mM PMSF, 1mg/ml aprotinin, 1mg/ml leupeptin, 1mg/ml pepstatin, 1mM Na3VO4, 1mM NaF) and diluted with non-reducing sample buffer (31mM Tris-HCl, pH 6.8, 5% glycerol, 1% SDS, 0.002% bromphenol blue).

Preparation Note

6 months at -20°C

Analysis Note

Routinely evaluated as a positive control for some of Upstates antibodies.

Legal Information

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

pictograms

Exclamation mark

signalword

Warning

hcodes

Hazard Classifications

Aquatic Chronic 3 - Eye Irrit. 2

Storage Class

12 - Non Combustible Liquids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

Regulatory Information

高风险级别生物产品-Merck
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Peixuan Zhu et al.
Leukemia & lymphoma, 54(3), 579-586 (2012-07-31)
Overexpression of zeta-chain-associated protein 70 (ZAP-70) was recently recognized as an independent prognostic marker for the aggressive form of chronic lymphocytic leukemia (CLL). The objective of this study was to demonstrate the feasibility and implementation of quantitative detection of ZAP-70
Gianluca Rosso et al.
International journal of bipolar disorders, 11(1), 33-33 (2023-10-09)
The differential diagnosis of patients presenting for the first time with a depressive episode into unipolar disorder versus bipolar disorder is crucial to establish the correct pharmacological therapy (antidepressants vs mood stabilizers), but no biological markers are currently available. Several
Apostolos Polykratis et al.
The Journal of biological chemistry, 280(23), 22454-22461 (2005-03-31)
Heparin affin regulatory peptide (HARP) is an 18-kDa secreted growth factor that has a high affinity for heparin and a potent role on tumor growth and angiogenesis. We have previously reported that HARP is mitogenic for different types of endothelial
Fumihiko Furuya et al.
Proceedings of the National Academy of Sciences of the United States of America, 103(6), 1780-1785 (2006-02-01)
Activation of the phosphatidylinositol 3-kinase (PI3K)-AKT/protein kinase B signaling pathway has been associated with multiple human cancers. Recently we showed that AKT is activated in both the thyroid and metastatic lesions of a mouse model of follicular thyroid carcinoma [thyroid
Sonia D'Souza et al.
Blood, 118(26), 6871-6880 (2011-11-02)
Protracted inhibition of osteoblast (OB) differentiation characterizes multiple myeloma (MM) bone disease and persists even when patients are in long-term remission. However, the underlying pathophysiology for this prolonged OB suppression is unknown. Therefore, we developed a mouse MM model in

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