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Proteinase K

Name: Proteinase K
Brand: MM

(from Tritirachium album) chromatographically purified lyophilized 30 mAnson-U/mg for biochemistry and molecular biology EC 3.4.21.14

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Synonym(s):

Proteinase K from Tritirachium album, Tritirachium alkaline proteinase, Endopeptidase K

CAS Number:

39450-01-6

Enzyme Commission number:

3.4.21.64 (BRENDA, IUBMB)

MDL number:

MFCD00132129

EC Index Number:

254-457-8

Quality Level

400

form

solid

mol wt

28.93 kDa

pH

6.2-6.8 (20 °C, 10 g/L in H₂O)

density

1.1 g/cm³

bulk density

35 kg/m³

storage temp.

2-8°C

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Application

Useful for the proteolytic inactivation of nucleases during the isolation of DNA and RNA.
Removes endotoxins that bind to cationic proteins such as lysozyme and ribonuclease A.
Reported useful for the isolation of hepatic, yeast, and mung bean mitochondria
Determination of enzyme localization on membranes
Treatment of paraffin embedded tissue sections to expose antigen binding sites for antibody labeling.
Digestion of proteins from brain tissue samples for prions in Transmissible Spongiform Encephalopathies (TSE) research.

Biochem/physiol Actions

Proteinase K is a stable and highly reactive serine protease. Evidence from crystal and molecular structure studies indicates the enzyme belongs to the subtilisin family with an active-site catalytic triad (Asp³⁹-His⁶⁹-Ser²²⁴). It is stable in a broad range of environments: pH, buffer salts, detergents (SDS), and temperature. In the presence of 0.1-0.5% SDS, proteinase K retains activity and will digest a variety of proteins and nucleases in DNA preparations without compromising the integrity of the isolated DNA.

Analysis Note

Appearance (color): white
Appearance (description): lyophilisate
Activity (hemoglobin; pH 7.5; 37 °C): ≥ 30.0 mAnsonU/mg
Spec. activity (calc. on protein): ≥ 40 mAnsonU/mg
DNases (Nicking activity; pBR 322; 6 h; 37 °C): not detectable
RNases (RNA; 2 h; 37°C): not detectable

Pictograms

GHS07,GHS08

Signal Word

Danger

Hazard Statements

H315 - H319 - H334 - H335

Precautionary Statements

P261 - P264 - P271 - P280 - P302 + P352 - P305 + P351 + P338

Hazard Classifications

Eye Irrit. 2 - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3

Target Organs

Respiratory system

Storage Class Code

11 - Combustible Solids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

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Correlation of conversion of Salmonella enterica serovar enteritidis phage type 1, 4, or 6 to phage type 7 with loss of lipopolysaccharide.

D L Baggesen et al.

Journal of clinical microbiology, 35(1), 330-333 (1997-01-01)

Studies of pairs of Salmonella enterica serovar Enteritidis isolates from different poultry flocks showed that phage type (PT) 7 may be derived from PT 1, 4, and 6. The conversion appeared to be associated with loss of lipopolysaccharide. It is

The alpha beta T cell receptor can replace the gamma delta receptor in the development of gamma delta lineage cells.

L Bruno et al.

Immunity, 5(4), 343-352 (1996-10-01)

In peripheral lymphoid tissues of TCR transgenic mice that express the nominal antigen (HY peptide plus H-2Db MHC) recognized by the transgenic TCR, there exist unusual CD4-CD8- and CD4-CD8low cells bearing the transgenic TCR. Here we show that, unlike TCR

Innate Lymphoid Cells in the Induction of Obesity.

Takaharu Sasaki et al.

Cell reports, 28(1), 202-217 (2019-07-04)

Complex interactions between immune cells are an important component in the induction of obesity. Here, we show that Il2rg-/-Rag2-/- mice lacking all lymphocytes are resistant to diet-induced obesity. Transplantation of bone marrow cells from Rag2-/- mice, which lack only acquired

Intergenerational effect of short-term spaceflight in mice.

Keisuke Yoshida et al.

iScience, 24(7), 102773-102773 (2021-07-20)

As space travel becomes more accessible, it is important to understand the effects of spaceflight including microgravity, cosmic radiation, and psychological stress. However, the effect on offspring has not been well studied in mammals. Here we investigated the effect of

A generic protocol for the affinity-purification of native macromolecular complexes from poxvirus-infected cells.

Julia Bartuli et al.

STAR protocols, 3(1), 101116-101116 (2022-02-05)

The functional and structural characterization of macromolecular complexes requires protocols for their native isolation. Here, we describe a protocol for this task based on the recombinant poxvirus Vaccinia expressing tagged proteins of interest in infected cells. Tagged proteins and their

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Articles

Proteinase K: Introduction & Applications

Proteinase K aids in molecular biology applications by digesting structural proteins, removing nucleases, and isolating intact genomic DNA.

How Proteinase K Can Be Used in Tissue Samples

The use of Pro K in combination with other reagents, such as detergents and chaotropic agents, can help to disrupt the cell membranes and release DNA from tissue. This is particularly important for downstream applications such as PCR, sequencing, and other molecular biology techniques that require pure and intact DNA.

How Proteinase K Can Be Used in Blood DNA Extraction Applications

In blood DNA extraction, Proteinase K, an enzyme commonly used to degrade proteins, can help break down the cellular and nuclear membranes, releasing DNA from the cells that protect it from degradation and increase purity/yield making it more suitable for various molecular biology techniques.

How to Determine the Best Product & Supplier for Proteinase K

The cost of proteinase K can vary depending on the source, purity, manufacturing process and vendor’s quality management system. It is important to balance the cost with the desired quality, performance, documentation and technical/quality support to select the optimal Proteinase K for the intended application

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