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Albumin, Bovine Serum, Fraction V, RIA and ELISA Grade

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Synonym(s):
Albumin, Bovine Serum, Fraction V, RIA and ELISA Grade
CAS Number:
9048-46-8

grade

RIA grade

Assay

≥98% (agarose gel electrophoresis)

form

solid

manufacturer/tradename

Calbiochem®

storage condition

OK to freeze

impurities

≤3.0 ash
≤5.0% moisture

color

off-white

solubility

0.15 M sodium chloride: 10 mg/mL

cation traces

heavy metals: ≤10 ppm

shipped in

ambient

storage temp.

2-8°C

Related Categories

General description

Albumin, Bovine Serum, Fraction V, RIA and ELISA Grade is a purified plasma protein that is useful as a stabilizing agent and as a carrier protein. It is prepared under non-denaturing conditions by modification of the Cohn procedure and caprylic acid heat-shock treatment. It has a pH of 6.8-7.4 at 10% in 150 mM NaCl.
Bovine serum albumin (BSA) is an α-helical, non-glycosylated globular protein with 17-disulfide bonds.

Application

Albumin, Bovine Serum, Fraction V, RIA and ELISA Grade has been used:
  • to block tissue sections of placenta in immunohistochemical (IHC) analysis
  • as a component of Krebs buffer for in vitro glucose-stimulated insulin secretion (GSIS) experiments and drug/palmitate preparation
  • as a vehicle control to incubate whole mouse blood to study plate aggregation in vivo

Biochem/physiol Actions

Bovine Serum Albumin (BSA), an important component in the cell culture media can transport fatty acids, hormones, and drugs. It is generally used as a blocking agent in enzyme-linked immunosorbent assay (ELISA).

Warning

Toxicity: Standard Handling (A)

Reconstitution

Following reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 6 months at -20°C.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Regulatory Information

监管及禁止进口产品

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