14-197-M
Casein Kinase 2 Protein, active, 10 µg
Active, recombinant full-length human Casein Kinase 2 protein, containing an α-subunit N-terminal 6His tag & a β-subunit N-terminal GST tag. For use in Kinase Assays.
Sign In to View Organizational & Contract Pricing.
Select a Size
Change View
About This Item
UNSPSC Code:
12352202
NACRES:
NA.41
eCl@ss:
32160405
Biological source:
human
Recombinant:
expressed in Sf21 cells
biological source
human
Quality Level
recombinant
expressed in Sf21 cells
mol wt
α-subunit Mw 49 kDa (heterotetramer; α2β2), β subunit Mw 53 kDa (heterotetramer; α2β2)
manufacturer/tradename
Upstate®
technique(s)
activity assay: suitable (kinase)
NCBI accession no.
UniProt accession no.
General description
Product Source: Expressed in Sf21 insect cells.
Recombinant full-length human Casein Kinase 2 protein, containing an &alpyha;-subunit N-terminal 6His tag and a β-subunit N-terminal GST tag.
Biochem/physiol Actions
Protein Target: CK2
Packaging
2 vials at 5μg each
Also available in 250μg size (2x125μg)--call for pricing and availability and reference catalog number 14-197M when ordering the 250μg size.
Also available in 250μg size (2x125μg)--call for pricing and availability and reference catalog number 14-197M when ordering the 250μg size.
Physical form
20mM MOPS, pH 7.4, 1mM EDTA, 0.1% (v/v) Triton X-100, 0.3% 2-mercaptoethanol, 1mM benzamidine, 50% glycerol.
Sequential chromatography using heparin Sepharose™ and Mono Q anion exchange resin
Preparation Note
1 year at -20°C.
Analysis Note
Routinely evaluated by phosphorylation of the Casein Kinase 2 Substrate Peptide.
Other Notes
For Specific Activity data, refer to the Certificate of Analysis for individual lots of this enzyme.
Legal Information
Sepharose is a trademark of Cytiva
UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
Already Own This Product?
Find documentation for the products that you have recently purchased in the Document Library.
In vitro phosphorylation of the polyomavirus major capsid protein VP1 on serine 66 by casein kinase II
Li, M., et al
The Journal of Biological Chemistry, 270, 26006-26011 (1995)
T Tiganis et al.
European journal of biochemistry, 229(3), 703-709 (1995-05-01)
Casein kinase II, cyclin-dependent kinases, and glycogen synthase kinase-3 are members of the protein kinase subfamily with a prominent insert in domain X of their catalytic subunit sequence. The function of the insert sequence in casein kinase II was investigated
Casein kinase II beta-subunit inhibits the activity of the catalytic alpha-subunit in the absence of salt.
Tiganis, T, et al.
Biochimica et Biophysica Acta, 1203, 282-289 (1993)
Global Trade Item Number
| SKU | GTIN |
|---|---|
| 14-197 | 04053252509773 |
| 14-197M | 04053252672484 |