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About This Item
UNSPSC Code:
12352202
eCl@ss:
32160405
NACRES:
NA.32
biological source
rat
Quality Level
mol wt
Mw 66 kDa
manufacturer/tradename
Upstate®
technique(s)
activity assay: suitable (kinase)
NCBI accession no.
UniProt accession no.
shipped in
dry ice
Gene Information
rat ... ROCK1(81762)
General description
N-terminal, His-tagged, fusion protein corresponding to amino acids 2-543 of rat ROKa/ROCK-II.
Note: The preparation contains numerous Sf21 cellular proteins which bind weakly to Ni-NTA agarose.
Note: The preparation contains numerous Sf21 cellular proteins which bind weakly to Ni-NTA agarose.
Product Source: Rat ROKa/ROCK-II expressed in Sf21 insect cells.
Biochem/physiol Actions
Protein Target: ROKα/ROCK-II
Target Sub-Family: AGC
Quality
Rroutinely evaluated using the S6 Kinase Assay Kit
Physical form
10μg of enzyme in 2.3μl of 50mM Tris/HCl pH7.5, 150mM NaCl, 0.1mM EGTA, 0.03% Brij-35, 270mM sucrose, 2mM benzamidine, 0.4mM PMSF, 0.1% 2-mercaptoethanol. Frozen solution.
Ni-NTA agarose
Storage and Stability
6 months at -70°C
Other Notes
For Specific Activity data, refer to the Certificate of Analysis for individual lots of this enzyme.
Legal Information
UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Signal Word
Warning
Hazard Statements
Precautionary Statements
Hazard Classifications
Skin Sens. 1
Storage Class Code
12 - Non Combustible Liquids
WGK
WGK 2
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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