14-440-M
PKA Protein, Recombinant, 10 µg
Recombinant human full length PKA, catalytic subunit type alpha, untagged, for use in Kinase Assays.
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About This Item
UNSPSC Code:
12352202
NACRES:
NA.41
eCl@ss:
32160405
Form:
liquid
Biological source:
human
Recombinant:
expressed in E. coli, expressed in BL21 DE3 cells
Mol wt:
Mw 40.7 kDa
biological source
human
Quality Level
recombinant
expressed in E. coli, expressed in BL21 DE3 cells
product line
Upstate®
form
liquid
specific activity
(For Specific Activity data, refer to the Certificate of Analysis for individual lots of this enzyme.), 8580 U/mg
shelf life
1 yr
mol wt
Mw 40.7 kDa
purified by
chromatography
species reactivity
human
manufacturer/tradename
Upstate®
technique(s)
activity assay: suitable (kinase)
solubility
water: soluble
accession no.
(FUNCTION: SwissProt: P17612 # Phosphorylates a large number of substrates in the cytoplasm and the nucleus. ), NM_002730.3
NCBI accession no.
UniProt accession no.
storage temp.
−70°C
Gene Information
human ... PRKACA(46909581)
General description
Research area: Cell Signaling
Product Source: Expressed in E.coli BL21 (DE3) pLysS cells Recombinant human full length PKA, catalytic subunit type alpha, untagged Protein kinase A (PKA) is a serine protein kinase and heterotetrameric holoenzyme that targets serine and threonine residues.
Product Source: Expressed in E.coli BL21 (DE3) pLysS cells Recombinant human full length PKA, catalytic subunit type alpha, untagged Protein kinase A (PKA) is a serine protein kinase and heterotetrameric holoenzyme that targets serine and threonine residues.
PKA
Recombinant human full length PKA, catalytic subunit type alpha, untagged
Biochem/physiol Actions
Protein Target: PKA; Target Sub-Family: AGC. Protein kinases are vital for integrating signaling networks within eukaryotic cells. In Hedgehog-responsive cells, PKA is crucial for regulating proliferation and fate determination by influencing Hedgehog signaling transduction. PKA has numerous targets that are linked to adhesion or migration.
Packaging
Also available in 10 μg size and in bulk
Also available in 250 µg size --call for pricing and availability and reference catalog number 14-440M when ordering the 250 µg size.
Physical form
Phosphocellulose P11 chromatography
Preparation Note
1 year at -70°C
Analysis Note
routinely evaluated by phosphorylation of Kemptide substrate in vitro
Other Notes
For Specific Activity data, refer to the Certificate of Analysis for individual lots of this enzyme.
Replaces: 14-114
Legal Information
UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage Class
10 - Combustible liquids
wgk
WGK 1
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
Already Own This Product?
Find documentation for the products that you have recently purchased in the Document Library.
Protein kinase A
Morrison H
Journal of Separation Science, 173-178 (2021)
PKA: a portrait of protein kinase dynamics
Taylor SS, et al.
Biochimica et Biophysica Acta, 1697(1-2), 259-269 (2004)
Prokaryotic expression of catalytic subunit of adenosine cyclic monophosphate-dependent protein kinase.
Yonemoto, W M, et al.
Test, 200, 581-596 (1991)
Global Trade Item Number
| SKU | GTIN |
|---|---|
| 14-440 | 04053252588150 |