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Merck
CN

14-476-M

Cdk7/Cyclin H/MAT1 (CAK complex) Protein, active, 10 µg

Active complex of recombinant full length human Cdk7, containing a C-terminal His6-tag, recombinant full-length human Cyclin H untagged & recombinant full-length human MAT1 containing an N-terminal GST-tag, for use in Kinase Assays.

Synonym(s):

CDKN7, Cell division protein kinase 7, MO15, STK1, Serine/threonine-protein kinase 1

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About This Item

UNSPSC Code:
12352202
NACRES:
NA.41
eCl@ss:
32160405

Key Documents

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Product Name

Cdk7/Cyclin H/MAT1 (CAK complex) Protein, active, 10 µg, Active complex of recombinant full length human Cdk7, containing a C-terminal His6-tag, recombinant full-length human Cyclin H untagged & recombinant full-length human MAT1 containing an N-terminal GST-tag, for use in Kinase Assays.

biological source

human

recombinant

expressed in baculovirus infected Sf21 cells

form

liquid

shelf life

1 yr

mol wt

Mw 37.8 kDa (cyclin H)
Mw 39.9 kDa (cdk7)
Mw 62.8 kDa (MAT1)

manufacturer/tradename

Upstate®

technique(s)

activity assay: suitable (kinase)

solubility

soluble

NCBI accession no.

NM_001799.2

UniProt accession no.

P50613

storage temp.

−70°C

Quality Level

100

Gene Information

human ... CDK7(1022)

Analysis Note

routinely evaluated by phosphorylation of cdk7 substrate peptide

Biochem/physiol Actions

The Cdk7/cyclin H/Mat1 (CAK complex) plays a role in both cell cycle regulation and transcription. Within the complex, Cdk7 functions as the Cdk-activating kinase for cell cycle and as a part of the general transcription factor, Transcription Factor II H (TFIIH), for transcription process. Additionally, it also regulates gene expression by phosphorylating transcription factors such as estrogen receptor-α (ER), p53, retinoid receptors, androgen receptor, and estrogen receptor. Elevated expression levels of Cdk7, cyclin H, and MAT1 have been observed in breast cancer patients. Inhibition of cdk7 is associated with the development of colorectal cancer in humans.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

Research area: APOPTOSIS

Protein Target: CDK7/cyclinH/MAT1 Target Sub-Family: CMGC

Complex of recombinant full length human cdk7, containing a C-terminal His6-tag, recombinant full-length human cyclin H untagged and recombinant full-length human MAT1 containing an N-terminal GST-tag Product Source: All are expressed by baculovirus in Sf21 insect cells

Cdk-activating kinase (CAK) is a trimeric complex composed of cyclin-dependent kinase 7 (Cdk7), cyclin H, and RING finger protein Methionine Adenosyltransferase 1 (MAT1). Cyclin H activates Cdk7, while MAT1 plays a role in influencing the substrate specificity of the complex.

Other Notes

For Specific Activity data, refer to the Certificate of Analysis for individual lots of this enzyme.

Packaging

Also available in 250μg size, please inquire for pricing and availability.

Physical form

Ni2+/NTA-agarose

Preparation Note

1 year at -70°C

Legal Information

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

pictograms

Exclamation mark
GHS07

signalword

Warning

hcodes

H317

Hazard Classifications

Skin Sens. 1

Storage Class

10 - Combustible liquids

wgk

WGK 2

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Functional analysis of the Cdk7.cyclin H.Mat1 complex in mouse embryonic stem cells and embryos
Shetal A Patel and M Celeste Simon
Journal of Separation Science, 285(20), 15587-15598 (2010)
DK regulators?Cell cycle progression or apoptosis?Scenarios in normal cells and cancerous cells
D'costa M, et al.
Advances in Protein Chemistry and Structural Biology, 135 (2023)
Expression of CDK7, Cyclin H, and MAT1 Is Elevated in Breast Cancer and Is Prognostic in Estrogen Receptor-Positive Breast Cancer
Patel H, et al.
Clinical Cancer Research, 22(23), 5929-5938 (2016)
Eric J Tomko et al.
Journal of molecular biology, 433(14), 166813-166813 (2021-01-17)
The general transcription factor TFIIH contains three ATP-dependent catalytic activities. TFIIH functions in nucleotide excision repair primarily as a DNA helicase and in Pol II transcription initiation as a dsDNA translocase and protein kinase. During initiation, the XPB/Ssl2 subunit of
Tinggui Yin et al.
Molecular cancer therapeutics, 13(6), 1442-1456 (2014-04-02)
DNA-dependent RNA polymerase II (RNAP II) largest subunit RPB1 C-terminal domain (CTD) kinases, including CDK9, are serine/threonine kinases known to regulate transcriptional initiation and elongation by phosphorylating Ser 2, 5, and 7 residues on CTD. Given the reported dysregulation of
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