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Merck
CN

14-601-M

ROKβ/ROCK-I Protein, active, 10 µg

Active, N-terminal His6-tagged, recombinant, human ROCK-I amino acids 17-535, autoactivated by incubating with Mg/ATP & redialysed to remove excess ATP. For use in Kinase Assays.

Synonym(s):

Rho-associated protein kinase, p160ROCK

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About This Item

UNSPSC Code:
12352202
NACRES:
NA.41
eCl@ss:
32160405

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Product Name

ROKβ/ROCK-I Protein, active, 10 µg, Active, N-terminal His6-tagged, recombinant, human ROCK-I amino acids 17-535, autoactivated by incubating with Mg/ATP & redialysed to remove excess ATP. For use in Kinase Assays.

biological source

human

recombinant

expressed in baculovirus infected Sf21 cells

form

liquid

shelf life

6 mo.

mol wt

Mw 61.4 kDa

manufacturer/tradename

Upstate®

technique(s)

activity assay: suitable (kinase)

NCBI accession no.

NM_005406.2

UniProt accession no.

Q13464

storage temp.

−70°C

Quality Level

100

Gene Information

human ... ROCK1(6093)

Analysis Note

routinely evaluated by phosphorylation of Long S6 substrate peptide (Catalog #12-420)

Application

Research Category
Inflammation & Immunology

Biochem/physiol Actions

G protein, RhoA/Rho kinase 1 (ROCK1) is a serine/threonine kinase. It plays a vital role in the regulation of cell growth, and invasive and metastatic progression of various types of human cancers. ROCK1 is involved in the regulation of apoptosis or programmed cell death in various cell types. Overexpression of ROCK1 has been observed in various tumors, including glioblastoma, melanoma, osteosarcoma, and hepatocellular carcinoma. This protein is known to enhance actin-myosin contractility by phosphorylating regulatory myosin light chains (MLCs). It also plays a role in cell motility through the modulation of integrin β1-activated focal adhesion kinase (FAK) signaling.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

Research area: Apoptosis

N-terminal His6-tagged, recombinant, human ROCK-I amino acids 17-535; autoactivated by incubating with Mg/ATP and redialysed to remove excess ATP

Product Source: expressed by baculovirus in Sf21 insect cells

Other Notes

For Specific Activity data, refer to the Certificate of Analysis for individual lots of this enzyme

Packaging

Also available in 250 µg size --call for pricing and availability and reference catalog number 14-601M when ordering the 250 µg size.

Physical form

Ni2+/NTA-agarose

Preparation Note

6 months at -70°C

Legal Information

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

pictograms

Exclamation mark
GHS07

signalword

Warning

hcodes

H317

Hazard Classifications

Skin Sens. 1

Storage Class

12 - Non Combustible Liquids

wgk

WGK 2

flash_point_f

Not applicable

flash_point_c

Not applicable

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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