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CN

1.46181

Rappaport Vassiliadis Medium

tube capacity 17 mL, tube filling volume 10 mL, closure type, with yellow cap, single packed of 20 tubes, for Salmonella spp.

Synonym(s):

Salmonella Enrichment Medium acc. to Rappaport and Vassiliadis, RVS Medium

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About This Item

NACRES:
NA.74
UNSPSC Code:
41106225
Feature:
closure type with yellow cap, ready-to-use
Agency:
EP 2.6.13, USP 62
Ph:
5.2

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agency

EP 2.6.13, USP 62

description

For the selective enrichment of samples for Salmonella sp. in accordance to EP

form

liquid (ready-to-use)

feature

closure type with yellow cap, ready-to-use

composition

with soy peptone

packaging

single packed of 20 tubes, tube of

technique(s)

direct inoculation: suitable, sterile filtration: suitable

tube capacity

17 mL

tube filling volume

10 mL

pH

5.2

Quality Level

300

application(s)

microbiology
pharmaceutical

storage temp.

2-25°C

suitability

Salmonella spp.

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General description

Rappaport Vassiliadis medium is used for the selective enrichment and presumptive detection of Salmonella sp. This medium is a modification of Rappaport-Vassiliadis enrichment broth and is used for motility enrichment in Petri dishes. It is a rapid and sensitive medium for the isolation of Salmonella sp. and can be seeded directly after pre-enrichment.

Application

Rappaport Vassiliadis medium is used for the selective enrichment and presumptive detection of Salmonella sp. in pharmaceutical products.

Analysis Note

Soy Peptone provides nitrogenous, carbonaceous, B complex vitamins and other essential growth nutrients. Phosphate provides buffering capacity to the medium. Malachite green serves as a selective agent. Sodium chloride maintains the osmotic equilibrium of the media. Salmonella generally survives a slightly high osmotic pressure (due to MgCl2 in the medium), grows at a slightly low pH, and is resistant to malachite green compared to other bacteria, thus making it a selective media for the growth of Salmonella sp.

Storage Class

10 - Combustible liquids

wgk

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable

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