1.46597
PPLO Broth
bottle capacity 1000 mL, bottle filling volume 500 mL, closure type, Blue screw cap with 3 loci, pack of 6 bottles, selective for mycoplasma
Synonym(s):
Mycoplasma Broth, Pleuropneumonia-like organisms (PPLO) Broth
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About This Item
UNSPSC Code:
12352201
Bottle capacity:
1000 mL
Bottle filling volume:
500 mL
Feature:
closure type Blue screw cap with 3 loci, ready-to-use
Agency:
EP 2.6.1, JP 4.06, USP 71
Ph:
7.6-8.0
Quality Level
material
glass bottle
agency
EP 2.6.1, JP 4.06, USP 71
sterility
sterile; autoclaved
form
liquid
shelf life
120 days
feature
closure type Blue screw cap with 3 loci, ready-to-use
composition
Beef Heart, Infusion, 6 g , NaCl, 5 g , Peptone, 10 g
packaging
pack of 6 bottles
technique(s)
direct inoculation: suitable
bottle capacity
1000 mL
bottle filling volume
500 mL
pH
7.6-8.0
application(s)
cosmetics
food and beverages
pharmaceutical
sterility testing
storage temp.
2-25°C
suitability
selective for mycoplasma
General description
PPLO Broth is a nutrient rich mycoplasma detection media. Mycoplasmas are the smallest bacteria besides nanobacteria. Due to the lack of cell wall structures they pass filters with pore sizes of 0.2 μm and show a great sensitivity towards osmotic changes within their habitats. They grow aerobically or under facultative anaerobic conditions.
This medium contains beef heart infusion and peptone to supply nutrients required for the growth of mycoplasmas. Sodium chloride maintains osmotic equilibrium. Yeast extract supplies a variety of B-complex vitamins and enhances growth. Normal horse serum provides a protein source. Penicillin and thallium acetate are added as inhibitors of bacteria. Yeasts and molds are inhibited by the amphotericin B contained in the medium.
This medium contains beef heart infusion and peptone to supply nutrients required for the growth of mycoplasmas. Sodium chloride maintains osmotic equilibrium. Yeast extract supplies a variety of B-complex vitamins and enhances growth. Normal horse serum provides a protein source. Penicillin and thallium acetate are added as inhibitors of bacteria. Yeasts and molds are inhibited by the amphotericin B contained in the medium.
Application
PPLO broth is used to detect presence of mycoplasma in test samples through direct inoculation or sterile filtration methods.
Other Notes
Production will take place at Millipore SAS in Molsheim, France. Deliveries will be executed in accordance with the terms and conditions of MLS RTU GmbH. A minimum order quantity is required to process the order. Once the order is placed and confirmed, Merck will ensure product delivery within a maximum timeframe of 10 to 12 weeks. The terms and conditions of Merck KGaA apply to both Millipore SAS and MLS RTU GmbH and can be accessed through the following link: Standard Terms & Conditions
This medium can be modified and/or supplemented based on the required performance criteria.
Disclaimer
This item is created at the customer′s request as a customized product and will not be developed or qualified in accordance with the Merck Product Development Process.
Storage Class
12 - Non Combustible Liquids
wgk
WGK 2
Regulatory Information
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Global Trade Item Number
| SKU | GTIN |
|---|---|
| 1465970006 | 04027269286019 |