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Merck
CN

14-688-M

PKG1α Protein, active, 10 µg

Active, N-terminal 6His-tagged, recombinant, full length PKG1α. For use in Kinase Assays.

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About This Item

UNSPSC Code:
12352202
NACRES:
NA.32
eCl@ss:
32160405

Key Documents

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Product Name

PKG1α Protein, active, 10 µg, Active, N-terminal 6His-tagged, recombinant, full length PKG1α. For use in Kinase Assays.

biological source

human

recombinant

expressed in baculovirus infected Sf21 cells

mol wt

Mw 80 kDa

manufacturer/tradename

Upstate®

technique(s)

activity assay: suitable (kinase)

NCBI accession no.

NM_001098512.1
NM_006258.2

UniProt accession no.

P14619

Quality Level

100

Gene Information

human ... PRKG1(5592)

Analysis Note

routinely evaluated by phosphorylation of PAKtide (RRRLSFAEPG).

Application

Research Category
Inflammation & Immunology

Biochem/physiol Actions

Protein Target: PKG1α
Target Sub-Family: AGC

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

N-terminal 6His-tagged, recombinant, full length PKG1α.
Product Source: PKG1α, expressed by baculovirus in Sf21 cells.

Other Notes

For Specific Activity data, refer to the Certificate of Analysis for individual lots of this enzyme.

Physical form

Ni2+/NTA-agarose

Preparation Note

6 months at -70°C from date of shipment

Legal Information

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

pictograms

Exclamation mark
GHS07

signalword

Warning

hcodes

H317

Hazard Classifications

Skin Sens. 1

Storage Class

12 - Non Combustible Liquids

wgk

WGK 2

flash_point_f

Not applicable

flash_point_c

Not applicable

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Mary G Johlfs et al.
PloS one, 10(7), e0132105-e0132105 (2015-07-02)
Profiling cellular proteome is critical to understanding signal integration during cell fate determination. In this study, the capability of capillary isoelectric focusing (cIEF) immunoassays to detect post-translational modifications (PTM) of protein isoforms is demonstrated. cIEF immunoassays exhibit protein detection sensitivity
Sequential activation of soluble guanylate cyclase, protein kinase G and cGMP-degrading phosphodiesterase is necessary for proper induction of long-term potentiation in CA1 of hippocampus. Alterations in hyperammonemia.
Monfort, Pilar, et al.
Neurochemistry International, 45, 895-901 (2004)
Modulation of synaptic function by cGMP and cGMP-gated cation channels.
Barnstable, Colin J, et al.
Neurochemistry International, 45, 875-884 (2004)
Physiology and pathophysiology of vascular signaling controlled by guanosine 3',5'-cyclic monophosphate-dependent protein kinase.
Birschmann, Ingvild and Walter, Ulrich
Acta Biochimica Polonica, 51, 397-404 (2004)
Zhenyu Li et al.
Cell, 112(1), 77-86 (2003-01-16)
It is currently accepted that cGMP-dependent protein kinase (PKG) inhibits platelet activation. Here, we show that PKG plays an important stimulatory role in platelet activation. Expression of recombinant PKG in a reconstituted cell model enhanced von Willebrand factor (vWF)-induced activation

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