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Merck
CN

14-742-M

FGFR2 (N549H) Protein, active, 10 µg

Active, N-terminal, 6His-tagged, recombinant, human FGFR2 (N549H), amino acids 456-770. For use in Kinase Assays.

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About This Item

UNSPSC Code:
12352202
NACRES:
NA.32
eCl@ss:
32160405

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Product Name

FGFR2 (N549H) Protein, active, 10 µg, Active, N-terminal, 6His-tagged, recombinant, human FGFR2 (N549H), amino acids 456-770. For use in Kinase Assays.

biological source

human

recombinant

expressed in baculovirus infected Sf21 cells

specific activity

6250 U/mg

mol wt

Mw 38.1 kDa

manufacturer/tradename

Upstate®

technique(s)

activity assay: suitable (kinase)

NCBI accession no.

NM_000141.3
NM_022970.2

UniProt accession no.

P21802

Quality Level

300

Gene Information

human ... FGFR2(2263)

Analysis Note

Routinely evaluated by phosphorylation of 500 μM GGEEEEYFELVKKKK.
Purity was assessed by SDS-PAGE and Coomassie blue staining using 3μg of active GFGR2 (N549H).

Application

Research Category
Metabolism
Research Sub Category
Cardiovascular Disease

Metabolic Disorders

Biochem/physiol Actions

Protein Target: FGFR2
Target Sub-Family: TK

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

N-terminal, 6His-tagged, recombinant, human FGFR2 (N549H), amino acids 456-770.
Product Source: recombinant, human FGFR2 (N549H), amino acids 456-770, expressed by baculovirus in Sf21 insect cells.

Other Notes

Specific Activity: where one unit of FGFR2 (N549H) activity is defined as 1nmol phosphate incorporated into 500μM GGEEEEYFELVKKKK per minute at 30°C with a final ATP concentration of 100µM.

Physical form

10 μg of enzyme in 6.3 μL of 50 mM Tris/HCl pH 7.5, 300 mM NaCl, 0.1 mM EGTA, 0.03% Brij 35, 270 mM sucrose, 1 mM benzamidine, 0.2 mM PMSF, 0.1% 2-mercaptoethanol. Frozen solution.
Ni2+/NTA-agarose

Preparation Note

Stable for 6 months at -70°C from date of shipment. For maximum recovery of product, centrifuge original vial prior to removing the cap.
Rapidly thaw the vial under cold water and immediately place on ice. Aliquot unused material into pre-chilled microcentrifuge tubes and immediately snap-freeze the vials in liquid nitrogen prior to re-storage at -70°C.

Legal Information

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

pictograms

Exclamation mark
GHS07

signalword

Warning

hcodes

H317

Hazard Classifications

Skin Sens. 1

Storage Class

10 - Combustible liquids

wgk

WGK 2

flash_point_f

Not applicable

flash_point_c

Not applicable

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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