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14-744-M

Sigma-Aldrich

Haspin Protein, active, 10 µg

Active, N-terminal 6His-tagged, recombinant, human Haspin, amino acids 471-end, expressed by baculovirus in Sf21 insect cells. For use in Kinase Assays.

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eCl@ss:
32160405
NACRES:
NA.32

biological source

human

Quality Level

100
300

recombinant

expressed in baculovirus infected Sf21 cells

specific activity

2237 U/mg

mol wt

Mw 41.1 kDa

manufacturer/tradename

Upstate®

technique(s)

activity assay: suitable (kinase)

NCBI accession no.

NM_031965.2

UniProt accession no.

Q8TF76

General description

N-terminal 6His-tagged, recombinant, human Haspin, amino acids 471-end, expressed by baculovirus in Sf21 insect cells.
Product Source: Recombinant enzyme expressed in Sf21 cells

Application

Research Category
Metabolism
Research Sub Category
Cardiovascular Disease

Metabolic Disorders

Biochem/physiol Actions

Protein Target: haspin
Target Sub-Family: Misc

Quality

Routinely evaluated by phosphorylation of 500 μM RARTLSFAEPG.
Purity was assessed by SDS-PAGE and Coomassie blue staining using 3μg of Haspin.

Unit Definition

Specific Activity: where one unit of Haspin activity is defined as 1 nmol phosphate incorporated into 500 μM RARTLSFAEPG per minute at 30°C with a final ATP concentration of 100 μM.

Physical form

10 μg of enzyme in 6.3 μL of 50 mM Tris/HCl pH 7.5, 300 mM NaCl, 0.1 mM EGTA, 0.03% Brij 35, 270 mM sucrose, 1 mM benzamidine, 0.2 mM PMSF, 0.1% 2-mercaptoethanol. Frozen solution.
Purified using Ni2+/NTA agarose. Purity 83% by SDS-PAGE and Coomassie blue staining.

Storage and Stability

Stable for 6 months at -70°C from date of shipment. For maximum recovery of product, centrifuge original vial prior to removing the cap.
Rapidly thaw the vial under cold water and immediately place on ice. Aliquot unused material into pre-chilled microcentrifuge tubes and immediately snap-freeze the vials in liquid nitrogen prior to re-storage at -70°C.

Legal Information

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Pictograms

Exclamation mark
GHS07

Signal Word

Warning

Hazard Statements

H317

Hazard Classifications

Skin Sens. 1

Storage Class Code

10 - Combustible liquids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

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