14-770-M
mTOR (1362-end) Protein, active, 10 µg
Active, N-terminal FLAG-tagged, recombinant, human mTOR, amino acids 1362-end, for use in Kinase Assays.
Synonym(s):
FRAP1
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About This Item
UNSPSC Code:
12352202
NACRES:
NA.41
eCl@ss:
32160405
Product Name
mTOR (1362-end) Protein, active, 10 µg, Active, N-terminal FLAG-tagged, recombinant, human mTOR, amino acids 1362-end, for use in Kinase Assays.
biological source
human
recombinant
expressed in baculovirus infected Sf21 cells
form
liquid
specific activity
377 U/mg
manufacturer/tradename
Upstate®
technique(s)
activity assay: suitable (kinase)
solubility
water: soluble
NCBI accession no.
UniProt accession no.
storage temp.
−70°C
Quality Level
Gene Information
human ... MTOR(2475)
Application
The active mTOR (1362-end) protein, 10 µg, has been used in the mTOR substrate phosphorylation assay to validate the biochemical and pharmacological profile of a novel mTOR-KI for targeting mTOR signaling pathways in vitro and in vivo.
Biochem/physiol Actions
Mechanistic target of rapamycin (mTOR) plays a pivotal role in regulating various essential cellular processes, ranging from protein synthesis to autophagy. Moreover, it influences metabolism, cellular survival, gene transcription, and cytoskeletal components. It gets activated in response to growth factors, nutrients, mitogens, and hormones.Downregulation of mTOR signaling is associated with the advancement of cancer and diabetes, and aging process.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
General description
Research area: Cell Signaling
Protein Target: mTOR (FRAP1)
Target Sub-Family: Lipid/Atypical
N-terminal FLAG-tagged, recombinant, human mTOR, amino acids 1362-end
The mechanistic target of rapamycin (mTOR) protein is a serine/threonine protein kinase belonging to PI3K-related kinase (PIKK) family, expressed throughout the body.
Protein Target: mTOR (FRAP1)
Target Sub-Family: Lipid/Atypical
N-terminal FLAG-tagged, recombinant, human mTOR, amino acids 1362-end
The mechanistic target of rapamycin (mTOR) protein is a serine/threonine protein kinase belonging to PI3K-related kinase (PIKK) family, expressed throughout the body.
Other Notes
Specific Activity: where one unit of mTOR activity is defined as 1 nmole of phosphate incorporated into 2mg/ml mTOR substrate per minute at 30°C with a final ATP concentration of 100µM.
Packaging
Also available in 100μg size, please inquire for pricing and availability.
Physical form
16µg of enzyme in 100µl of 10mM HEPES, 50mM NaCl, 50mM β-glycerophosphate, 0.25mM sodium orthovanadate, 10mM NaF, 300µg/ml FLAGTM peptide, 20% v/v glycerol
Preparation Note
Store -70°C. For maximum recovery of product, centrifuge original vial prior to removing the cap.
Legal Information
UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany
Storage Class
10 - Combustible liquids
wgk
WGK 2
Certificates of Analysis (COA)
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Find documentation for the products that you have recently purchased in the Document Library.
mTOR Signaling in Growth, Metabolism, and Disease
Saxton RA and Sabatini DM
Cells, 168(6), 960?976- 960?976 (2017)
mTOR: Exploring a New Potential Therapeutic Target for Stroke
Castellanos M, et al.
Molecules to Medicine with mTOR, 105-122 (2016)
Jianchang Qian et al.
Oncotarget, 7(41), 67071-67086 (2016-08-27)
The mechanistic target of rapamycin (mTOR) is a rational target for cancer treatment. While the mTORC1-selective rapalogs have shown significant benefits in the clinic, antitumor response may be further improved by inhibiting both mTORC1 and mTORC2. Herein, we established target
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