NAP1 Protein, active, 300 µg

N-terminal 6×His-tagged Drosophila NAP1 expressed in E. coli.

Nucleosome assembly proteins (NAP) are involved in moving histones into the nucleus, nucleosome assembly and chromatin fluidity. They affect the transcription of many genes.

Lot # R0803D0002: 12.5 µg NAP1 has been successfully used for 1 chromatin assembly reaction with Chromatin Assembly Kit (Millipore Corp, Catalog# 17-410).

10 mM K-HEPES pH 7.6, 10 mM KCl, 50 mM NaCl, 0.1 mM EDTA, 10%(v/v) glycerol, 0.01% NP-40, 0.2 mM PMSF, 1mM DTT. Frozen solution.

Format: Purified

Stable for 6 months at -80°C from date of shipment. For maximum recovery of product, centrifuge original vial prior to removing the cap.

Activity is routinely tested with Chromatin Assembly Kit (Millipore Corp, Catalog# 17-410).

Specific Activity: Varies with lot

Varies with individual lot. Lot R0803D0002 has purity 90% by SDS-PAGE and Coomassie blue staining

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