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Merck
CN

14-837

NAP1 Protein, active, 300 µg

Active N-terminal His6-tagged Drosophila NAP1 expressed in E. coli.

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关于此项目

UNSPSC Code:
12352202
NACRES:
NA.32
eCl@ss:
32160405
Mol wt:
Mw 43 kDa

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mol wt

Mw 43 kDa

manufacturer/tradename

Upstate®

UniProt accession no.

Q9W1G7

shipped in

dry ice

Quality Level

100

Gene Information

Drosophila ... NAP1(37798)

General description

N-terminal 6×His-tagged Drosophila NAP1 expressed in E. coli.
Nucleosome assembly proteins (NAP) are involved in moving histones into the nucleus, nucleosome assembly and chromatin fluidity. They affect the transcription of many genes.

Application

Lot # R0803D0002: 12.5 µg NAP1 has been successfully used for 1 chromatin assembly reaction with Chromatin Assembly Kit (Millipore Corp, Catalog# 17-410).

Physical form

10 mM K-HEPES pH 7.6, 10 mM KCl, 50 mM NaCl, 0.1 mM EDTA, 10%(v/v) glycerol, 0.01% NP-40, 0.2 mM PMSF, 1mM DTT. Frozen solution.
Format: Purified

Preparation Note

Stable for 6 months at -80°C from date of shipment. For maximum recovery of product, centrifuge original vial prior to removing the cap.

Analysis Note

Activity is routinely tested with Chromatin Assembly Kit (Millipore Corp, Catalog# 17-410).

Other Notes

Specific Activity: Varies with lot
Varies with individual lot. Lot R0803D0002 has purity 90% by SDS-PAGE and Coomassie blue staining

Legal Information

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

存储类别

10 - Combustible liquids

wgk

WGK 2

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Sangeeta Chandrashekar et al.
Molecules (Basel, Switzerland), 26(7) (2021-05-01)
The study aimed to investigate the antibacterial activity of Mustard (Brassica juncea) and Moringa (Moringa oleifera) leaf extracts and coagulant protein for their potential application in water treatment. Bacterial cell aggregation and growth kinetics studies were employed for thirteen bacterial
Roberto Fabian et al.
Molecules (Basel, Switzerland), 26(16) (2021-08-28)
We report data from single molecule studies on the interaction between single DNA molecules and core histones using custom-designed horizontal magnetic tweezers. The DNA-core histone complexes were formed using λ-DNA tethers, core histones, and NAP1 and were exposed to forces

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Shaping Epigenetics Discovery - Epigenetics Product Selection Brochure

"Epigenetics describes heritable changes in gene expression caused by non-genetic mechanisms instead of by alterations in DNA sequence. These changes can be cell- or tissue-specific, and can be passed on to multiple generations. Epigenetic regulation enriches DNAbased information, allowing a cell to vary its response across diverse biological and environmental contexts. Although epigenetic mechanisms are primarily centered in the nucleus, these mechanisms can be induced by environmental signals such as hormones, nutrients, stress, and cellular damage, pointing to the involvement of cytoplasmic and extracellular factors in epigenetic regulation."

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