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Merck
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14-840-M

AMPK (α1, β1, γ1) Protein, active, 10 µg

Active, recombinant, human AMPK (α1, β1, γ1) holoenzyme, comprised of full length N-terminal 6His-tagged AMPKα1, full length untagged AMPKβ1 & full length untagged AMPKγ1. For use in Kinase Assays.

Synonym(s):

AMPK kinase

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About This Item

UNSPSC Code:
12352202
NACRES:
NA.32
eCl@ss:
32160405

Key Documents

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Product Name

AMPK (α1, β1, γ1) Protein, active, 10 µg, Active, recombinant, human AMPK (α1, β1, γ1) holoenzyme, comprised of full length N-terminal 6His-tagged AMPKα1, full length untagged AMPKβ1 & full length untagged AMPKγ1. For use in Kinase Assays.

biological source

human

recombinant

expressed in E. coli

form

liquid

shelf life

6 mo.

mol wt

Mw 30 kDa (AMPKß1)
Mw 38 kDa (AMPKγ1)
Mw 64 kDa (AMPKα1)

manufacturer/tradename

Upstate®

technique(s)

activity assay: suitable (kinase)

solubility

water: soluble

GenBank accession no.

AF100763

NCBI accession no.

NM_002733
NM_006253

UniProt accession no.

P54619
Q13131
Q9Y478

storage temp.

−70°C

Quality Level

100

Gene Information

human ... PRKAB1(5564)

Analysis Note

Routinely evaluated by phosphorylation of substrate peptide

Application

For use in Kinase Assays.
Research Category
Metabolism

Apoptosis & Cancer
Research Sub Category
Diabetes

Obesity

Metabolic Disorders

Biochem/physiol Actions

Under conditions of nutrient scarcity, AMPK functions as a metabolic checkpoint and inhibits cellular growth. Upon activation, AMPK promotes glucose uptake and fatty acid oxidation thereby reducing energy-consuming processes like protein and lipid synthesis. Furthermore, AMPK regulates autophagy and mitophagy. It also facilitates catabolic pathways to generate more ATP as a response to decreased ATP production.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

Research area: Cell Signaling

Product Source: Expressed in E. coli cells and activated in vivo.

Protein Target: AMPK (α1, β1, γ1)

Target Sub-Family: CAMK

Recombinant, human AMPK (α1, β1, γ1) holoenzyme, comprised of full- length N-terminal 6His-tagged AMPKα1, full length untagged AMPKβ1 and full length untagged AMPKγ1. The AMPK α catalytic subunit in mammals is encoded by α1 and α2, two β genes β1 and β2 and three γ subunit genes γ1, γ2, and γ3.

Other Notes

For Specific Activity data, refer to the Certificate of Analysis for individual lots of this enzyme.

Packaging

Also available in 250µg size as catalog # 14-840M - Call for pricing and availability.

Physical form

Cobalt-agarose

Preparation Note

6 months at -70°C

Legal Information

GenBank is a registered trademark of United States Department of Health and Human Services
UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

pictograms

Exclamation mark
GHS07

signalword

Warning

hcodes

H317

Hazard Classifications

Skin Sens. 1

Storage Class

12 - Non Combustible Liquids

wgk

WGK 2

flash_point_f

Not applicable

flash_point_c

Not applicable

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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AMPK and the biochemistry of exercise: Implications for human health and disease
Richter EA and Ruderman NB
The Biochemical Journal, 418(2), 261?275-261?275 (2009)
AMPK: guardian of metabolism and mitochondrial homeostasis
Herzig S and Shaw RJ
Molecular Cell Biology, 19, 121?135-121?135 (2018)
The AMP-activated protein kinase (AMPK) signaling pathway coordinates cell growth, autophagy, & metabolism
Mihaylova MM and Shaw RJ
Nature Cell Biology, 13(9), 1016?1023-1016?1023 (2011)

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