14-934-M
GCN2, active
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About This Item
UNSPSC Code:
12352200
NACRES:
NA.32
eCl@ss:
32160405
biological source
human
mol wt
Mw 215 kDa
manufacturer/tradename
Upstate®
technique(s)
activity assay: suitable (kinase)
NCBI accession no.
UniProt accession no.
Quality Level
Gene Information
human ... EIF2AK4(440275)
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
General description
GCN2 (EIF2AK4) belongs to a family of kinases that phosphorylate the alpha subunit of eukaryotic translation initiation factor-2 (EIF2S1) to downregulate protein synthesis in response to varied cellular stresses.
Product Source: N-terminal GST-tagged, recombinant, human GCN2 full length, expressed by baculovirus in Sf21 insect cells. The recombinant protein contains the amino acid substitution E556G with reference to GenPept NP_001013725. E556G is reported in GenPept CAH10626.
Analysis Note
Routinely evaluated by phosphorylation of peptide RSRSRSRSRSRSRSR
Biochem/physiol Actions
Target Sub-Family: STE
Other Notes
For Specific Activity data, refer to the Certificate of Analysis for individual lots of this enzyme.
Packaging
Also available in 250μg size as catalog # 14-934M and in bulk as catalog # 14-934-K.
Physical form
Protein in 50mM Tris/HCl pH7.5, 300mM NaCl, 0.1mM EGTA, 0.03% Brij-35, 270mM sucrose, 1mM benzamidine, 0.2mM PMSF, 0.1% 2-mercaptoethanol. Frozen solution.
Purified using glutathione sepharose.
Preparation Note
6 months at -70°C
Legal Information
UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany
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Warning
hcodes
Hazard Classifications
Skin Sens. 1
Storage Class
12 - Non Combustible Liquids
wgk
WGK 2
flash_point_f
Not applicable
flash_point_c
Not applicable
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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