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Protein A Agarose, Fast Flow

Protein A Agarose, Fast Flow suitable for medium and low-pressure chromatography, immunoprecipitation and antibody purification.

Synonym(s):

Protein A resin

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About This Item

UNSPSC Code:
41116133
NACRES:
NA.56
eCl@ss:
32160801
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form

liquid

manufacturer/tradename

Upstate®

technique(s)

affinity chromatography: suitable, immunoprecipitation (IP): suitable, western blot: suitable

shipped in

wet ice

General description

Protein A is an immunoglobulin (Ig)-binding protein used to purify large amounts of IgG. It binds to the Fc part of the antibody at the CH2–CH3 interface. Protein A-agarose might be suitable for low-pressure antibody isolation.
Recombinant Protein A covalently coupled to highly cross-linked 6% agarose beads.

Binding capacity: 40mg human IgG/ml agarose

Application

Protein A Agarose, Fast Flow has been used in immunoprecipitation and chromatin immunoprecipitation (ChIP).

Physical form

sterile distilled water containing 0.01% thimerosal

Analysis Note

routinely evaluated in immunoprecipitation

Legal Information

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

For U.S. Customers: Contains mercury; Do not place in trash - dispose according to local, state, or federal laws.

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Storage Class

10 - Combustible liquids

wgk

WGK 1

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Nature, 515(7527), 371-375 (2014-11-21)
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Vasavi Sundaram et al.
Genome research, 24(12), 1963-1976 (2014-10-17)
Transposable elements (TEs) have been shown to contain functional binding sites for certain transcription factors (TFs). However, the extent to which TEs contribute to the evolution of TF binding sites is not well known. We comprehensively mapped binding sites for
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Genome research, 17(6), 898-909 (2007-06-15)
Recent progress in mapping transcription factor (TF) binding regions can largely be credited to chromatin immunoprecipitation (ChIP) technologies. We compared strategies for mapping TF binding regions in mammalian cells using two different ChIP schemes: ChIP with DNA microarray analysis (ChIP-chip)
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Global Trade Item Number

SKUGTIN
16-15604053252409752