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Merck
CN

16-157

Protein A Agarose/Salmon Sperm DNA, 2.5 mL

for use in chromatin immunoprecipitations (ChIP assays)

Synonym(s):

ChIP agarose beads, ChIP agaraose A beads, ChIP assays

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About This Item

UNSPSC Code:
12352202
NACRES:
NA.32
eCl@ss:
32160405
Form:
liquid
Biological source:
Staphylococcus aureus
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biological source

Staphylococcus aureus

Quality Segment

form

liquid

manufacturer/tradename

Upstate®

technique(s)

ChIP: suitable

suitability

suitable for immunoprecipitation

shipped in

wet ice

General description

Protein A covalently bound to agarose by alkylamine linkage containing sonicated Salmon Sperm DNA. The salmon sperm DNA blocks non-specific interactions with the beads for use in chromatin IP (ChIP) assays. Note part number 16-157C present in the ChIP kits is not available separately, please purchase 16-157.
Protein A from Staphylococcus aureus has a very high affinity for the Fc regions of IgG molecules. Immoblisation of protein A on beads creates an affinity resin which can be used to purify IgG fractions from crude serum, ascites fluid or cell cuture media.

Application

Suitable for use in chromatin immunoprecipitation (ChIPs) assays

Physical form

10mM Tris-HCl, 1mM EDTA, pH 8.0 containing 0.05% sodium azide.

Analysis Note

Routinely evaluated in a Chromatin Immunoprecipitation by pulling down DNA cross-linked to acetylated histones; subsequent detection was performed using anti-Acetyl Histone H4, ChIPs Grade (06-866)

Legal Information

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany


Storage Class

12 - Non Combustible Liquids

wgk

WGK 1

Regulatory Information

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Global Trade Item Number

SKUGTIN
16-15704053252515330