16-282
Anti-Phosphotyrosine Antibody, clone 4G10®, Magnetic Bead Conjugate
clone 4G10®, from mouse
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About This Item
UNSPSC Code:
12352203
NACRES:
NA.41
eCl@ss:
32160702
Product Name
Anti-Phosphotyrosine Antibody, clone 4G10®, Magnetic Bead Conjugate, clone 4G10®, from mouse
biological source
mouse
antibody form
purified antibody
antibody product type
primary antibodies
clone
4G10®, monoclonal
species reactivity (predicted by homology)
all
technique(s)
immunoprecipitation (IP): suitable
isotype
IgG2bκ
shipped in
wet ice
target post-translational modification
phosphorylation (pTyr)
Quality Level
Gene Information
human ... PID1(55022)
Analysis Note
100 μg of NIH3T3 whole cell lysate stimulated with 100 ng/mL PDGF (10 minutes) was immunoprecipitated (IP) using either anti-mouse IgG (Cat. No. 12-371) (lane 1), or Anti-Phosphotyrosine, Magnetic Bead Conjugate
(Lane 2).
(Lane 2).
Control
NIH3T3 whole cell lysate stimulated with 50 ng/mL PDGF (5 minutes)
NIH3T3 whole cell lysate stimulated with 50 ng/mL PDGF (5 minutes)
Application
Detect Phosphotyrosine using this Anti-Phosphotyrosine Antibody, clone 4G10, Magnetic Bead Conjugate validated for use in IP.
Research Category
Signaling
Signaling
Research Sub Category
General Post-translation Modification
General Post-translation Modification
Biochem/physiol Actions
This antibody recognizes tyrosine-phosphorylated proteins from all species
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
General description
Dependent upon the molecular weight of the tyrosine phosphorylated protein being detected.
Tyrosine phosphorylation is considered as one of the key steps in signal transduction and regulation of enzymatic activity. Some of the tyrosine residues can be tagged with a phosphate group (phosphorylated) by protein kinases. (In its phosphorylated state, it is referred to as phosphotyrosine.).The advent of anti-phosphotyrosine antibodies is one of significant events in signal transduction research. Before the availability of anti-phosphotyrosine antibodies, tyrosyl phospyhorylation of proteins and enzymes was investigated through hazardous and time-consuming radioactive experiments. Anti-phosphotyrosine antibodies are commonly used in western blots after the targeted proteins have been immunoprecipitated to measure the tyrosyl phosphorylation of the proteins. Anti-phosphotyrosine antibodies are also directly used on cell lysate to examine the overall change of tyrosine phosphorylation level in reponse to various treatments.
Immunogen
Phosphotyramine-KLH
Physical form
20% slurry; 1xPBS, 0.05% sodium azide. Sufficient for 10 IPs.
Format: Purified
Protein G-Sepharose Chromatography
Preparation Note
Stable for 1 year at 2-8ºC from date of receipt.
Note: DO NOT FREEZE
Note: DO NOT FREEZE
Legal Information
4G10 is a registered trademark of Upstate Group, Inc.
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Storage Class
12 - Non Combustible Liquids
wgk
WGK 2
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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