Sign In to View Organizational & Contract Pricing.
Select a Size
About This Item
UNSPSC Code:
12161503
NACRES:
NA.84
eCl@ss:
32161000
manufacturer/tradename
Upstate®
technique(s)
activity assay: suitable (kinase)
NCBI accession no.
UniProt accession no.
shipped in
wet ice
Quality Level
Application
MAP Kinase/Erk Assay Kit is designed to measure the phosphotransferase activity of MAP (mitogen-activated protein) Kinase in immunoprecipitates & column fractions.
Used to detect/quantify: MAP Kinase/Erk
Analysis Note
The assay kit is designed to measure the phosphotransferase activity of MAP (mitogen-activated protein) Kinase in immunoprecipitates and column fractions. The assay kit is based on phosphorylation of a specific substrate (myelin basic protein, MBP). The phosphorylated substrate is then analyzed by immunoblot analysis, probing with a monoclonal Phospho-specific MBP antibody.
Other Notes
Assay Dilution Buffer I (ADBI) (Cat.# 20-108)
MAP Kinase/Erk Substrate Cocktail II (Cat.# 20-166)
Inhibitor Cocktail (Cat.# 20-116)
Magnesium/ATP Cocktail (Cat.# 20-113)
Anti-phospho-MBP, clone P12 (Cat.# 05-429)
MAP Kinase/Erk Substrate Cocktail II (Cat.# 20-166)
Inhibitor Cocktail (Cat.# 20-116)
Magnesium/ATP Cocktail (Cat.# 20-113)
Anti-phospho-MBP, clone P12 (Cat.# 05-429)
This product is derived from bovine source. Export of this product to certain countries may be restricted. Please contact Customer Service or your local distributor to inquire about product availability and export options.
Legal Information
UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage Class
12 - Non Combustible Liquids
flash_point_f
Not applicable
flash_point_c
Not applicable
Regulatory Information
新产品
This item has
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
Already Own This Product?
Find documentation for the products that you have recently purchased in the Document Library.
K B Andersson et al.
European journal of immunology, 26(12), 3170-3178 (1996-12-01)
CD22 is a B cell-restricted surface molecule which may play an important role in interactions between B cells and other cells and in regulating signals through the B cell receptor (BCR) complex. Here we have examined whether the mouse is
Madhukar Varshney et al.
Analytical chemistry, 80(6), 2141-2148 (2008-02-15)
Nanomechanical resonators have shown potential application for mass sensing and have been used to detect a variety of biomolecules. In this study, a dynamic resonance-based technique was used to detect prion proteins (PrP), which in conformationally altered forms are known
M Zanetti et al.
The Journal of biological chemistry, 269(11), 7855-7858 (1994-03-18)
A group of myeloid precursors of defense peptides has recently been shown to have highly homologous N-terminal regions. Using a strategy based on this homology, a novel cDNA was cloned from pig bone marrow RNA and found to encode a
D Carré-Eusèbe et al.
The Biochemical journal, 277 ( Pt 1), 39-45 (1991-07-01)
Protamine P2, the major basic chromosomal protein of mouse spermatozoa, is synthesized as a precursor almost twice as long as the mature protein, its extra length arising from an N-terminal extension of 44 amino acid residues. This precursor is integrated
Matilde Marchi et al.
PloS one, 3(12), e3873-e3873 (2008-12-05)
The Extracellular Regulated Kinase 1 and 2 transduce a variety of extracellular stimuli regulating processes as diverse as proliferation, differentiation and synaptic plasticity. Once activated in the cytoplasm, ERK1 and ERK2 translocate into the nucleus and interact with nuclear substrates
Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.
Contact Technical Service