178615
Anti-Aquaporin 5 Rabbit pAb
liquid, Calbiochem®
Synonym(s):
Anti-AQP5
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About This Item
NACRES:
NA.43
UNSPSC Code:
12352203
Clone:
polyclonal
Species reactivity:
rat, mouse, human
Application:
—
Citations:
24
biological source
rabbit
Quality Level
antibody form
affinity isolated antibody
antibody product type
primary antibodies
clone
polyclonal
form
liquid
does not contain
preservative
species reactivity
rat, mouse, human
manufacturer/tradename
Calbiochem®
storage condition
OK to freeze, avoid repeated freeze/thaw cycles
dilution
(ELISA (0.5-1.0 µg/mL)
Immunoblotting (1-10 µg/mL)
Immunofluorescence (2-20 µg/mL)
Immunohistochemistry (2-20 µg/mL))
isotype
IgG
shipped in
wet ice
storage temp.
−20°C
target post-translational modification
unmodified
General description
Anti-Aquaporin 5, rabbit polyclonal, recognizes the ~28 kDa AQP 5 protein in epithelial tissue extracts. It is validated for ELISA, Western blotting, immunofluorescence, and immunohistochemistry.
Immunoaffinity purified rabbit polyclonal antibody. Recognizes the ~28 kDa AQP5 protein.
Recognizes the ~28 kDa AQP 5 protein in epithelial tissue extracts.
Immunogen
a synthetic peptide corresponding to a 17-amino acid sequence in the cytoplasmic region of rat AQP5
Application
ELISA (0.5-1.0 µg/ml)
Immunoblotting (1-10 µg/ml)
Immunofluorescence (2-20 µg/ml)
Immunohistochemistry (2-20 µg/ml)
Immunoblotting (1-10 µg/ml)
Immunofluorescence (2-20 µg/ml)
Immunohistochemistry (2-20 µg/ml)
Packaging
Please refer to vial label for lot-specific concentration.
Analysis Note
Positive Control
Epithelial cells
Epithelial cells
Other Notes
Does not cross-react with other AQP proteins. The use of 0.5-1.0% milk in all primary/secondary antibody-enzyme conjugate incubations is recommended in order to suppress non-specific bands. Variables associated with assay conditions will dictate the optimal working dilution.
Lee, M.D., et al. 1996. J. Biol. Chem. 271, 8599.
Raina, S., et al. 1995. J. Biol. Chem. 270, 1908.
Raina, S., et al. 1995. J. Biol. Chem. 270, 1908.
Legal Information
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Toxicity: Standard Handling (A)
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Storage Class
10 - Combustible liquids
wgk
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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M Cristina Kenney et al.
The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society, 52(10), 1341-1350 (2004-09-24)
Corneas with edema-related diseases lose transparency, which causes significant vision loss. This study analyzed seven aquaporins (AQPs) in normal corneas, pseudophakic/aphakic bullous keratopathy (PBK/ABK) corneas, Fuchs' dystrophy corneas, keratoconus corneas, post-cataract surgery (PCS) corneas, and normal organ-cultured corneas. RNA levels
Amy P Wong et al.
The Journal of clinical investigation, 119(2), 336-348 (2009-01-24)
The bone marrow compartment is enriched in stem and progenitor cells, and an unidentified subpopulation of these cells can contribute to lung epithelial repair. Here we identify this subpopulation and quantitate its relative contribution to injured airway epithelium. A subpopulation
Ninche Ninche et al.
Development (Cambridge, England), 147(19) (2020-10-01)
Salivary glands exert exocrine secretory function to provide saliva for lubrication and protection of the oral cavity. Its epithelium consists of several differentiated cell types, including acinar, ductal and myoepithelial cells, that are maintained in a lineage-restricted manner during homeostasis
Global Trade Item Number
| SKU | GTIN |
|---|---|
| 178615-50UG | 04055977204926 |