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197217

Bcl-xL BH4₄₋₂₃

Name: Bcl-xL BH4₄₋₂₃
Brand: MM

≥95% (HPLC), voltage-dependent anion channel blocker, lyophilized

Synonym(s):

Bcl-x_L BH4₄₋₂₃, Human, Cell-Permeable, HIV-TAT_48-57-β-Ala-Bcl-x_L BH4_4-23, Ac-GRKKRRQRRR-βA-SNRELVVDFLSYKLSQKGYS-OH

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About This Item

Empirical Formula (Hill Notation):

C₁₆₅H₂₇₉N₅₉O₄₆

Molecular Weight:

3825.35

UNSPSC Code:

12352202

NACRES:

NA.77

Assay:

≥95% (HPLC)

Form:

lyophilized

Storage condition:

OK to freeze, desiccated (hygroscopic)

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Properties

Product Name

Bcl-x_L BH4₄₋₂₃, Human, Cell-Permeable, A cell-permeable peptide that prevents apoptotic cell death by directly binding to the voltage-dependent anion channel (VDAC) and blocking its activity.

Quality Segment

100

assay

≥95% (HPLC)

form

lyophilized

manufacturer/tradename

Calbiochem^®

storage condition

OK to freeze, desiccated (hygroscopic)

solubility

20% acetic acid: 5 mg/mL, DMSO: 5 mg/mL

shipped in

ambient

storage temp.

−20°C

Description

General description

A cell-permeable peptide that prevents apoptotic cell death by directly binding to the voltage-dependent anion channel (VDAC) and blocking its activity. Leads to the inhibition of cytochrome c release and loss of mitochondrial membrane potential (ΔΨ_m). Contains the conserved N-terminal homology domain (BH4) of Bcl-x_L (amino acids 4 - 23) that has been shown to be essential for inhibiting VDAC activity in liposomes and in isolated mitochondria. The BH4 domain is linked to a carrier peptide, a 10-amino acid HIV-TAT_48-57 sequence with a β-alanine residue as a spacer for maximum flexibility. Following its uptake, it is mainly localized to the mitochondria.

A highly cell-permeable peptide that prevents apoptotic cell death by directly binding to the voltage-dependent anion channel (VDAC) and blocking its activity. Ultimately results in the inhibition of cytochrome c release and loss of mitochondrial membrane potential (ΔΨm). Contains the conserved N-terminal homology domain (BH4) of Bcl-x_L (amino acids 4-23) that has been shown to be essential for inhibiting VDAC activity in liposomes and isolated mitochondria. The BH4 domain is linked to the carrier peptide, the 10-amino acid HIV-TAT_48-57 sequence with a β-alanine residue as a spacer for maximum flexibility. Following its uptake, it is mainly localized in the mitochondria.

Biochem/physiol Actions

Cell permeable: yes

Primary Target
Voltage-dependent anion channel (VDAC)

Product does not compete with ATP.

Reversible: yes

Packaging

Packaged under inert gas

Physical form

Supplied as a trifluoroacetate salt.

Preparation Note

Following reconstitution aliquot and freeze (-20°C). Stock solutions are stable for up to 3 months at -20°C.

Other Notes

Ac-Gly-Arg-Lys-Lys-Arg-Arg-Gln-Arg-Arg-Arg-βAla-Ser-Asn-Arg-Glu-Leu-Val-Val-Asp-Phe-Leu-Ser-Tyr-Lys-Ser-Gln-Lys-Gly-Tyr-Ser-OH

Chen m., et al. 2002. J. Biol. Chem. 277, 29181.
Futaki, S., et al. 2001. J. Biol. Chem.276, 5836.
Shimizu, S., et al. 2000. Proc. Natl. Acad. Sci. USA97, 3100.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Toxicity: Standard Handling (A)

Storage Class

11 - Combustible Solids

wgk

WGK 1

flash_point_f

Not applicable

flash_point_c