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203189

Biotin-X-NHS, Water-Soluble

Name: Biotin-X-NHS, Water-Soluble
Brand: MM

Synonym(s):

Biotin-X-NHS, Water-Soluble, Sulfobiotin-X-NHS, Sulfosuccinimidyl-6-(biotinamido)hexanoate

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About This Item

Empirical Formula (Hill Notation):

C₂₀H₂₉N₄O₉S₂ · Na

Molecular Weight:

556.59

NACRES:

NA.41

UNSPSC Code:

41116133

Assay:

≥94% (HPLC)

Form:

solid

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Properties

Quality Level

100

biological source

(Synthetic chemical)

assay

≥94% (HPLC)

form

solid

manufacturer/tradename

Calbiochem^®

storage condition

OK to freeze, desiccated

color

off-white to tan

solubility

DMSO: 13 mg/mL, distilled water: 5.5 mg/mL

shipped in

ambient

storage temp.

2-8°C

Description

General description

Readily couples biotin to primary amino groups of proteins under mild alkaline conditions. Ideally suited for labeling antibodies, hormones, or other polypeptide molecules containing lysine residues. Extended spacer arm provides maximum accessibility of biotin for avidin conjugates.

Water solubility is extremely convenient, eliminating the need to dissolve the reagent in an organic solvent such as dimethylformamide (DMF) and dimethylsulfoxide (DMSO) prior to use.

Preparation Note

Unstable in aqueous solution; reconstitute just prior to use. Following reconstitution in DMSO, Aliquot and freeze (-20°C). DMSO stock solutions are stable for up to 1 month -20°C.

Other Notes

Gretch, D.R., et al. 1987. Anal. Biochem.163, 270.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Toxicity: Standard Handling (A)

Storage Class

11 - Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c