208748
Calpain-1 Substrate, Fluorogenic
An internally quenched fluorogenic substrate peptide derived from the calpain-1 cleavage site of α-spectrin.
Synonym(s):
Calpain-1 Substrate, Fluorogenic, μ-Calpain Substrate, Fluorogenic, H-K(FAM)-EVY~GMMK(DABCYL)-OH
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About This Item
Empirical Formula (Hill Notation):
C79H95N13O19S2
Molecular Weight:
1594.81
NACRES:
NA.77
UNSPSC Code:
12352200
Assay:
≥95% (HPLC)
Form:
lyophilized solid
Quality level:
Storage condition:
OK to freeze, desiccated (hygroscopic), protect from light
Quality Level
assay
≥95% (HPLC)
form
lyophilized solid
manufacturer/tradename
Calbiochem®
storage condition
OK to freeze, desiccated (hygroscopic), protect from light
color
red
solubility
DMSO: 1 mg/mL
fluorescence
λex ~490 nm, λem ~518 nm
shipped in
wet ice
storage temp.
−20°C
General description
An internally quenched fluorogenic substrate peptide derived from the calpain-1 cleavage site of α-spectrin. It is not recognized by trypsin or α-chymotrypsin and serves as a sensitive and specific substrate for calpain-1 (Km = 4.6 µM; kcat = 11 s-1). Cleavage occurs at the Tyr-Gly bond and results in enhanced fluorescence. Excitation max: ~490 nm; emission max: ~518 nm.
Biochem/physiol Actions
Cell permeable: no
Primary Target
A sensitive and specific substrate for calpain-1
A sensitive and specific substrate for calpain-1
Product does not compete with ATP.
Reversible: no
Packaging
Packaged under inert gas
Physical form
Supplied as a trifluoroacetate salt.
Preparation Note
Following reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 3 months at -20°C.
Other Notes
H-Lys(FAM)-Glu-Val-Tyr~Gly-Met-Met-Lys(DABCYL)-OH
Mittoo, S., et al. 2003. Anal. Biochem.319, 234.
Legal Information
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Toxicity: Standard Handling (A)
Storage Class
11 - Combustible Solids
wgk
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
Certificates of Analysis (COA)
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David S Dang et al.
Meat science, 162, 108039-108039 (2020-01-15)
The purpose of this study was to examine the role of mitochondria in postmortem calcium homeostasis and its effect on proteolysis and tenderness. We hypothesized that mitochondria buffer cytosolic calcium levels and delay the activation of calpain-1 and subsequently the
Global Trade Item Number
| SKU | GTIN |
|---|---|
| 208748-2MG | 04055977202830 |