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Merck
CN

208912

Anti-Calreticulin Mouse mAb (FMC75)

liquid, clone FMC75, Calbiochem®

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About This Item

NACRES:
NA.43
UNSPSC Code:
12352203

Key Documents

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Product Name

Anti-Calreticulin Mouse mAb (FMC75), liquid, clone FMC75, Calbiochem®

biological source

mouse

antibody form

purified antibody

antibody product type

primary antibodies

clone

FMC75, monoclonal

form

liquid

does not contain

preservative

species reactivity

human, monkey

should not react with

rat, E. coli, canine, hamster, salmon, bovine, mouse, guinea pig

manufacturer/tradename

Calbiochem®

storage condition

OK to freeze
avoid repeated freeze/thaw cycles

dilution

(Enzyme Immunoassay (0.1 µg/mL)
Immunoblotting (0.5 µg/mL)
Immunocytochemistry
Immunohistochemistry
Flow Cytometry )

isotype

IgG1

shipped in

wet ice

storage temp.

−20°C

target post-translational modification

unmodified

Quality Level

100

Analysis Note

Positive Control
Human Molt 4, MCF-7, HeLa, K562, A431, Jurkat, Hs67, Hs198 cells, or monkey Vero cells

Application

Enzyme Immunoassay (0.1 µg/ml)

Immunoblotting (0.5 µg/ml, colorimetric; see application references)

Immunocytochemistry (see application references)

Immunohistochemistry (see application references)

Flow Cytometry (see application references)

Disclaimer

Toxicity: Standard Handling (A)

General description

Anti-Calreticulin, mouse monoclonal, clone FMC75, recognizes the ~63 kDa calreticulin in Molt 4, MCF-7, HeLa, K562, A431 & other cells. It is validated for FC, WB, ICC, IHC, and in enzyme immunoassay.
Protein G purified mouse monoclonal antibody. Recognizes the ~63 kDa (apparent MW) calreticulin protein.
Recognizes the ~63 kDa (apparent MW) calreticulin protein in Molt 4, MCF-7, HeLa, K562, A431, Jurkat, Hs67, Hs198, and vero cells.

Immunogen

Human
a recombinant protein consisting of human calreticulin fused to maltose binding protein

Other Notes

Does not cross-react with GrpE or Grp78. Variables associated with assay conditions will dictate the proper working dilution.
Nauseef, W.M., et al. 1995. J. Biol. Chem. 270, 4741.
Jethmalani, S.M., et al. 1994. J. Biol. Chem. 269, 23603.
Smith, M.J., and Koch, G.L.E. 1989. EMBO J.8, 3581.

Packaging

Please refer to vial label for lot-specific concentration.

Physical form

In PBS, 50% Glycerol and 0.09% Sodium Azide.

Preparation Note

Following initial thaw, aliquot and freeze (-20°C).

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

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Storage Class

10 - Combustible liquids

wgk

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Stephanie S Lam et al.
Nature methods, 12(1), 51-54 (2014-11-25)
APEX is an engineered peroxidase that functions as an electron microscopy tag and a promiscuous labeling enzyme for live-cell proteomics. Because limited sensitivity precludes applications requiring low APEX expression, we used yeast-display evolution to improve its catalytic efficiency. APEX2 is
Mutation-Driven S100A8 Overexpression Confers Aberrant Phenotypes in Type 1 CALR-Mutated MPN.
Wang, et al.
International Journal of Molecular Sciences, 24 (2023)
Ying Yin et al.
PLoS biology, 16(8), e2005140-e2005140 (2018-08-08)
Some secreted proteins that assemble into large complexes, such as extracellular matrices or hormones and enzymes in storage granules, must be kept at subaggregation concentrations during intracellular trafficking. We show surfeit locus protein 4 (Surf4) is the cargo receptor that

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