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About This Item

UNSPSC Code:
12352203
NACRES:
NA.43
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biological source

mouse

Quality Level

100

antibody form

purified antibody

antibody product type

primary antibodies

clone

FMC75, monoclonal

form

liquid

does not contain

preservative

species reactivity

human, monkey

should not react with

rat, E. coli, canine, hamster, salmon, bovine, mouse, guinea pig

manufacturer/tradename

Calbiochem®

storage condition

OK to freeze
avoid repeated freeze/thaw cycles

dilution

(Enzyme Immunoassay (0.1 µg/mL)
Immunoblotting (0.5 µg/mL)
Immunocytochemistry
Immunohistochemistry
Flow Cytometry )

isotype

IgG1

shipped in

wet ice

storage temp.

−20°C

target post-translational modification

unmodified

General description

Anti-Calreticulin, mouse monoclonal, clone FMC75, recognizes the ~63 kDa calreticulin in Molt 4, MCF-7, HeLa, K562, A431 & other cells. It is validated for FC, WB, ICC, IHC, and in enzyme immunoassay.
Protein G purified mouse monoclonal antibody. Recognizes the ~63 kDa (apparent MW) calreticulin protein.
Recognizes the ~63 kDa (apparent MW) calreticulin protein in Molt 4, MCF-7, HeLa, K562, A431, Jurkat, Hs67, Hs198, and vero cells.

Immunogen

Human
a recombinant protein consisting of human calreticulin fused to maltose binding protein

Application

Enzyme Immunoassay (0.1 µg/ml)

Immunoblotting (0.5 µg/ml, colorimetric; see application references)

Immunocytochemistry (see application references)

Immunohistochemistry (see application references)

Flow Cytometry (see application references)

Packaging

Please refer to vial label for lot-specific concentration.

Physical form

In PBS, 50% Glycerol and 0.09% Sodium Azide.

Preparation Note

Following initial thaw, aliquot and freeze (-20°C).

Analysis Note

Positive Control
Human Molt 4, MCF-7, HeLa, K562, A431, Jurkat, Hs67, Hs198 cells, or monkey Vero cells

Other Notes

Does not cross-react with GrpE or Grp78. Variables associated with assay conditions will dictate the proper working dilution.
Nauseef, W.M., et al. 1995. J. Biol. Chem. 270, 4741.
Jethmalani, S.M., et al. 1994. J. Biol. Chem. 269, 23603.
Smith, M.J., and Koch, G.L.E. 1989. EMBO J.8, 3581.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Toxicity: Standard Handling (A)

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Storage Class Code

10 - Combustible liquids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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