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Merck
CN

216254

BCECF/AM

A variable mixture of cell-permeable ester derivatives of BCECF that are hydrolyzed by cytosolic esterases to yield intracellularly trapped indicator BCECF.

Synonym(s):

BCECF/AM, 2ʹ,7ʹ- bis-(Carboxyethyl)-5(6ʹ)-carboxyfluorescein Acetoxymethyl Ester, 2ʹ,7ʹ-bis-(Carboxyethyl)-5(6ʹ)-carboxyfluorescein Acetoxymethyl Ester

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About This Item

UNSPSC Code:
12352200
NACRES:
NA.25
MDL number:
MFCD00036969

Key Documents

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Product Name

BCECF/AM, A variable mixture of cell-permeable ester derivatives of BCECF that are hydrolyzed by cytosolic esterases to yield intracellularly trapped indicator BCECF.

SMILES string

O1C3(c4c(cc(c(c4)CCC(=O)OCOC(=O)C)OCOC(=O)C)Oc5c3cc(c(c5)OCOC(=O)C)CCC(=O)OCOC(=O)C)c2c(cc(cc2)C(=O)OCOC(=O)C)C1=O

InChI key

NTECHUXHORNEGZ-UHFFFAOYSA-N

assay

≥85% (mixture of isomers, HPLC)

form

solid

manufacturer/tradename

Calbiochem®

storage condition

OK to freeze
protect from light

color

white to yellow

solubility

acetonitrile: 25 mg/mL
DMF: soluble
DMSO: soluble

fluorescence

λex 500-504 nm
λem 525-531 nm

shipped in

ambient

storage temp.

−20°C

Quality Level

100

Related Categories

Biochem/physiol Actions

Cell permeable: yes
Primary Target
Monitor intracellular pH changes
Product does not compete with ATP.
Reversible: no

Disclaimer

Toxicity: Irritant (B)

General description

A variable mixture of cell-permeable ester derivatives of BCECF (Cat. No. 216252) that are hydrolyzed by cytosolic esterases to yield intracellularly trapped indicator BCECF. Has been used to monitor intracellular pH changes in mammalian fibroblasts, gastric cells, lymphocytes, myocytes, and distal convoluted tubules. Typical loading buffer contains 1-10 µM BCECF/AM in serum-free medium that is free of amino acids and buffers containing primary or secondary amines.

Other Notes

Futsaetner, C.M., et al. 1993. Can. J. Microbiol. 39, 180.
Hayashi, H., et al. 1992. Am. J. Physiol. 262, C628.
Sasaki, S., et al. 1992. Biochim. Biophys. Acta 1137, 45.
Miyata, H., et al. 1989. Biochem. Biophys. Res. Commun. 163, 500.
Weiner, I.D., and Hamm, L.L. 1989. Am. J. Physiol.256, F957.

Packaging

Packaged under inert gas

Preparation Note

Following reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 1 week at -20°C.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Storage Class

11 - Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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