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216254

BCECF/AM

Name: BCECF/AM
Brand: MM

A variable mixture of cell-permeable ester derivatives of BCECF that are hydrolyzed by cytosolic esterases to yield intracellularly trapped indicator BCECF.

Synonym(s):

BCECF/AM, 2ʹ,7ʹ- bis-(Carboxyethyl)-5(6ʹ)-carboxyfluorescein Acetoxymethyl Ester, 2ʹ,7ʹ-bis-(Carboxyethyl)-5(6ʹ)-carboxyfluorescein Acetoxymethyl Ester

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About This Item

UNSPSC Code:

12352200

NACRES:

NA.25

MDL number:

MFCD00036969

Assay:

≥85% (mixture of isomers, HPLC)

Form:

solid

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Properties

Quality Level

100

assay

≥85% (mixture of isomers, HPLC)

form

solid

manufacturer/tradename

Calbiochem^®

storage condition

OK to freeze, protect from light

color

white to yellow

solubility

acetonitrile: 25 mg/mL, DMF: soluble, DMSO: soluble

fluorescence

λ_ex 500-504 nm, λ_em 525-531 nm

shipped in

ambient

storage temp.

−20°C

SMILES string

O1C3(c4c(cc(c(c4)CCC(=O)OCOC(=O)C)OCOC(=O)C)Oc5c3cc(c(c5)OCOC(=O)C)CCC(=O)OCOC(=O)C)c2c(cc(cc2)C(=O)OCOC(=O)C)C1=O

InChI key

NTECHUXHORNEGZ-UHFFFAOYSA-N

Description

General description

A variable mixture of cell-permeable ester derivatives of BCECF (Cat. No. 216252) that are hydrolyzed by cytosolic esterases to yield intracellularly trapped indicator BCECF. Has been used to monitor intracellular pH changes in mammalian fibroblasts, gastric cells, lymphocytes, myocytes, and distal convoluted tubules. Typical loading buffer contains 1-10 µM BCECF/AM in serum-free medium that is free of amino acids and buffers containing primary or secondary amines.

Biochem/physiol Actions

Cell permeable: yes

Primary Target
Monitor intracellular pH changes

Product does not compete with ATP.

Reversible: no

Packaging

Packaged under inert gas

Preparation Note

Following reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 1 week at -20°C.

Other Notes

Futsaetner, C.M., et al. 1993. Can. J. Microbiol. 39, 180.
Hayashi, H., et al. 1992. Am. J. Physiol. 262, C628.
Sasaki, S., et al. 1992. Biochim. Biophys. Acta 1137, 45.
Miyata, H., et al. 1989. Biochem. Biophys. Res. Commun. 163, 500.
Weiner, I.D., and Hamm, L.L. 1989. Am. J. Physiol.256, F957.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Toxicity: Irritant (B)

Storage Class

11 - Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c