216275-M
5-Carboxyfluorescein Diacetate
Vital stain. Non-fluorescent, hydrophobic agent that permeates intact cell membranes. In the cell, it is enzymatically hydrolyzed to a hydrophilic agent, 5-Carboxyfluorescein that exhibits strong fluorescence.
Synonym(s):
5-Carboxyfluorescein Diacetate, 5-CFDA
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About This Item
Quality Segment
assay
≥95% (HPLC)
form
solid
manufacturer/tradename
Calbiochem®
storage condition
OK to freeze, desiccated, protect from light
color
yellow
solubility
DMSO: 5 mg/mL
storage temp.
−20°C
SMILES string
O1C3(c4c(cc(cc4)OC(=O)C)Oc5c3ccc(c5)OC(=O)C)c2c(cc(cc2)C(=O)O)C1=O
InChI
1S/C25H16O9/c1-12(26)31-15-4-7-19-21(10-15)33-22-11-16(32-13(2)27)5-8-20(22)25(19)18-6-3-14(23(28)29)9-17(18)24(30)34-25/h3-11H,1-2H3,(H,28,29)
InChI key
WPUZGNPQMIWOHE-UHFFFAOYSA-N
General description
Vital stain. Non-fluorescent, hydrophobic agent that permeates intact cell membranes. In the cell, it is enzymatically hydrolyzed to a hydrophilic agent, strongly fluorescent 5-Carboxyfluorescein (Cat. No. 216265).
Biochem/physiol Actions
Primary Target
Vital stain
Vital stain
Preparation Note
Following reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 3 months at -20°C.
Other Notes
Bruning, J.W., et al. 1980. J. Immunol. Methods33, 33.
Legal Information
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Toxicity: Standard Handling (A)
Storage Class
11 - Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
Certificates of Analysis (COA)
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