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Merck
CN

217594

Anti-CD44 Rat mAb (A020)

liquid, clone A020, Calbiochem®

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About This Item

NACRES:
NA.41
UNSPSC Code:
12352203

Key Documents

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Product Name

Anti-CD44 Rat mAb (A020), liquid, clone A020, Calbiochem®

biological source

rat

antibody form

purified antibody

antibody product type

primary antibodies

clone

A020, monoclonal

form

liquid

contains

≤0.1% sodium azide as preservative

species reactivity

canine, bovine, human, rabbit, porcine, mouse

manufacturer/tradename

Calbiochem®

storage condition

OK to freeze
avoid repeated freeze/thaw cycles

dilution

(ELISA (1:2000)
Flow Cytometry (1:1000)
Immunoblotting (1:1000)
Immunofluorescence (1:200)
Immunohistochemistry (1:250)
Immunoprecipitation (1:200)
Neutralization Studies
Radioimmunoassay (1:5000))

isotype

IgG2b

shipped in

wet ice

storage temp.

−20°C

target post-translational modification

unmodified

Quality Level

100

Gene Information

bovine ... Cd44(281057)
dog ... Cd44(403939)
human ... CD44(960)
mouse ... Cd44(12505)
pig ... Cd44(100525150)

Application

ELISA (1:2000)

Flow Cytometry (1:1000)

Immunoblotting (1:1000)

Immunofluorescence (1:200)

Immunohistochemistry (1:250)

Immunoprecipitation (1:200)

Neutralization Studies (see comments and application references)

Radioimmunoassay (1:5000)

Disclaimer

Toxicity: Standard Handling (A)

General description

Anti-CD44, rat monoclonal, clone A020, recognizes the ~85-95 kDa CD44 protein (gp90) in human and GP85 in mouse. It is validated for ELISA, IF, IP, RIA, WB, and IHC.
Rat monoclonal antibody purified by ammonium sulfate precipitation and DEAE chromatography. Recognizes the ~85-95 kDa CD44 protein.
Recognizes the ~85-95 kDa CD44 protein (gp90) in human and GP85 or Pgp-1 in mouse.

Immunogen

Human
purified human lymphocyte CD44

Other Notes

Iida, N., and Bourguignon, L.Y.W. 1995. J. Cell Physiol.162, 121.
Bourguignon, L.Y.W., et al. 1993. J. Immunol.151, 6634.
The antibody specifically recognizes and reacts with a common determinant of the CD44 class of glycoproteins. This antibody has been reported to block the interaction between hyaluronan and CD44 (see application references). Variables associated with assay conditions will dictate the proper working dilution.

Packaging

Please refer to vial label for lot-specific concentration.

Physical form

In 150 mM NaCl, 10 mM sodium phosphate, pH 7.5.

Preparation Note

Following initial thaw, aliquot and freeze (-20°C).

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

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Storage Class

12 - Non Combustible Liquids

wgk

nwg

flash_point_f

Not applicable

flash_point_c

Not applicable

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Shibnath Ghatak et al.
The Journal of biological chemistry, 280(10), 8875-8883 (2005-01-06)
Hyaluronan is enriched in many types of human cancers, and manipulations of hyaluronan expression or interactions have a major influence on tumor progression in animal models. Increased ErbB2 activity is characteristic of several cancers and is responsible for many aspects
Jay J Cao et al.
Journal of bone and mineral research : the official journal of the American Society for Bone and Mineral Research, 20(1), 30-40 (2004-12-28)
HA activates CD44 to stimulate RANKL expression in bone marrow stromal cells. HA stimulation of RANKL is blocked by anti-CD44 antibody and is absent in cells from CD44(-/-) mice. CD44(-/-) mice exhibit thicker cortical bone and a smaller medullary cavity
Daniel S Puperi et al.
Biomacromolecules, 17(5), 1766-1775 (2016-04-28)
Advanced tissue engineered heart valves must be constructed from multiple materials to better mimic the heterogeneity found in the native valve. The trilayered structure of aortic valves provides the ability to open and close consistently over a full human lifetime
Eun-Ju Chang et al.
Journal of cell science, 120(Pt 1), 166-176 (2006-12-14)
The differentiation of osteoclasts, cells specialized for bone resorption, is governed by two key factors, macrophage colony stimulating factor (M-CSF) and receptor activator of nuclear factor kappaB ligand (RANKL). The extracellular matrix (ECM) is an important factor influencing cell fate.
Mengya Zhao et al.
Cell reports, 42(9), 113038-113038 (2023-08-25)
Chronic neurodegeneration and acute injuries lead to neuron losses via diverse processes. We compared retinal ganglion cell (RGC) responses between chronic glaucomatous conditions and the acute injury model. Among major RGC subclasses, αRGCs and intrinsically photosensitive RGCs (ipRGCs) preferentially survive
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