recombinant
expressed in E. coli
Quality Level
Assay
≥70% (SDS-PAGE)
form
lyophilized
specific activity
≥400 units/mg protein
manufacturer/tradename
Calbiochem®
storage condition
OK to freeze
solubility
PBS with 15% glycerol: 1 units/μL
shipped in
wet ice
storage temp.
−70°C
General description
Recombinant, human caspase-9 fused at the N- and C- termini to a His•Tag sequence and expressed in E. coli as a single polypeptide. Subsequently undergoes spontaneous autoprocessing to yield the active enzyme. MW: 11000 and 20000.
Physical form
Lyophilized from 130 mM NaCl, 20 mM Tris, 4 mM (NH₄)₂SO₄
Preparation Note
Following reconstitution, aliquot and freeze (-70°C). Avoid freeze/thaw cycles of solutions. Stock solutions are stable for up to 4 months at -20°C.
Other Notes
One unit is defined as the amount of enzyme that will cleave 1.0 nmol substrate Ac-LEHD-pNA (Cat. No. 218805) per h at 37°C, pH 7.2.
Qin, H., et al. 1999. Nature399, 549.
Li, P., et al. 1997. Cell91, 479.
Salvesen, G.S., et al. 1997. Cell91, 443.
Li, P., et al. 1997. Cell91, 479.
Salvesen, G.S., et al. 1997. Cell91, 443.
Legal Information
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Toxicity: Standard Handling (A)
Storage Class Code
11 - Combustible Solids
WGK
WGK 1
Certificates of Analysis (COA)
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