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218807

Sigma-Aldrich

Caspase-9, Human, Recombinant, E. coli

Caspase-9, Human, Recombinant is expressed in E. coli as a single polypeptide. Fused at the N- and C- termini to a His•Tag sequence. Undergoes spontaneous autoprocessing to yield the active enzyme.

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Synonym(s):
ICE-LAP6, Mch6

recombinant

expressed in E. coli

Quality Level

100

Assay

≥70% (SDS-PAGE)

form

lyophilized

specific activity

≥400 units/mg protein

manufacturer/tradename

Calbiochem®

storage condition

OK to freeze

solubility

PBS with 15% glycerol: 1 units/μL

shipped in

wet ice

storage temp.

−70°C

General description

Recombinant, human caspase-9 fused at the N- and C- termini to a His•Tag sequence and expressed in E. coli as a single polypeptide. Subsequently undergoes spontaneous autoprocessing to yield the active enzyme. MW: 11000 and 20000.

Warning

Toxicity: Standard Handling (A)

Unit Definition

One unit is defined as the amount of enzyme that will cleave 1.0 nmol substrate Ac-LEHD-pNA (Cat. No. 218805) per h at 37°C, pH 7.2.

Physical form

Lyophilized from 130 mM NaCl, 20 mM Tris, 4 mM (NH₄)₂SO₄

Reconstitution

Following reconstitution, aliquot and freeze (-70°C). Avoid freeze/thaw cycles of solutions. Stock solutions are stable for up to 4 months at -20°C.

Other Notes

Qin, H., et al. 1999. Nature399, 549.
Li, P., et al. 1997. Cell91, 479.
Salvesen, G.S., et al. 1997. Cell91, 443.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Storage Class Code

11 - Combustible Solids

WGK

WGK 1

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