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CHAPS, Molecular Biology Grade

CAS 75621-03-3 is a zwitterionic detergent that combines features of bile salts and N-alkyl sulfobetaines.

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Synonym(s):
CHAPS, 3-[(3-Cholamidopropyl)dimethylammonio]-1-propanesulfonate
Empirical Formula (Hill Notation):
C32H58N2O7S
CAS Number:
75621-03-3
Molecular Weight:
614.88
MDL number:
MFCD00012116

grade

Molecular Biology

Quality Level

300

Assay

≥98% (NMR)

form

crystalline powder

mol wt

micellar avg mol wt 6150

manufacturer/tradename

Calbiochem®

storage condition

OK to freeze
desiccated (hygroscopic)

aggregation number

10

color

white

CMC

6 - 10 mM
6 mM (20-25°C)

transition temp

cloud point >100 °C

solubility

water: 1.0 M

foreign activity

DNase, RNase, and Protease, none detected

shipped in

ambient

storage temp.

15-25°C

SMILES string

C[C@H](CCC(=O)NCCC[N+](C)(C)CCCS([O-])(=O)=O)[C@H]1CC[C@H]2[C@@H]3[C@H](O)CC4C[C@H](O)CC[C@]4(C)[C@H]3C[C@H](O)[C@]12C

InChI

1S/C32H58N2O7S/c1-21(8-11-29(38)33-14-6-15-34(4,5)16-7-17-42(39,40)41)24-9-10-25-30-26(20-28(37)32(24,25)3)31(2)13-12-23(35)18-22(31)19-27(30)36/h21-28,30,35-37H,6-20H2,1-5H3,(H-,33,38,39,40,41)/t21-,22?,23-,24-,25+,26+,27-,28+,30+,31+,32-/m1/s1

InChI key

UMCMPZBLKLEWAF-RFCNGIAKSA-N

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General description

Conductivity (1.0 M, 24°C): <100 µmhos.
Non-denaturing zwitterionic detergent that combines features of bile salts and N-alkyl sulfobetaines. Better at solubilizing proteins than the structurally related carboxylic acid anions, and is more effective at breaking protein-protein interactions than either sodium cholate or TRITON™ X-100. Capable of solubilizing the opiate receptor to a state exhibiting reversible binding to opiates. Also capable of disaggregating P 450 to its monomeric form without denaturation. Has a small micellar molecular weight (6150 Daltons) and a high critical micellar concentration (6-10 mM). Can be removed from either gels or protein solutions by dialysis. Aggregation number: 4-14.
Zwitterionic detergent that combines features of bile salts and N-alkyl sulfobetaines. Better at solubilizing proteins than structurally-related carboxylic acid anions and is much more effective at breaking protein-protein interactions than either sodium cholate or TRITON™ X-100 Detergent. Capable of solubilizing the opiate receptor to a state exhibiting reversible binding of opiates. Also, capable of disaggregating cytochrome P450 to its monomeric form without denaturation. Has a small micellar molecular weight and a high critical micellar concentration and can be removed from either gels or protein solutions by dialysis across a membrane. Aggregation number: 4-14.

Application

CHAPS is a nondenaturing zwitterionic detergent for membrane biochemistry.
Useful for solubilizing membrane proteins and breaking protein-protein interactions. CHAPS′ small micellar molecular weight (6,150) and high critical micelle concentration (6-10 mM) allow it to be removed from samples by dialysis. It is also suitable for protein solubilization for isoelectric focusing and two-dimensional electrophoresis. CHAPS is commonly used for non-denaturing (without urea) IEF and has been shown to give excellent resolution of some subcellular preparations and plant proteins. Concentrations between 2-4% (w/v) are typically used in an IEF gel.

Warning

Toxicity: Harmful (C)

Reconstitution

Following reconstitution, store in the refrigerator (4°C). Stock solutions are stable for up to 6 months at 4°C.

Other Notes

Chen, Y., et al. 1992. Biochemistry 31, 2415.
Ofri, D., et al. 1992. J. Neurochem. 58, 628.
Ransom, R.W., et al. 1992. Biochem. Pharmacol. 43, 1823.
Simonds, W.F., et al. 1992. Proc. Natl. Acad. Sci. USA77, 4623.
Yannariello-Brown, J., and Weigel, P.H. 1992. Biochemistry31, 576.
Hjelmeland, L.M. 1980. Proc. Natl. Acad. Sci. USA77, 6368.
Simonds, W.F., et al. 1980. Proc. Natl. Acad. Sci. USA77, 4623.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
Triton is a trademark of The Dow Chemical Company or an affiliated company of Dow

Pictograms

Exclamation mark
GHS07

Signal Word

Warning

Hazard Statements

H302

Precautionary Statements

P264 - P270 - P301 + P312 - P501

Hazard Classifications

Acute Tox. 4 Oral

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

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