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230914

Sigma-Aldrich

Chymotrypsin Substrate II, Fluorogenic

Sensitive fluorogenic substrate for the quantitative determination of chymotrypsin activity.

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Synonym(s):
Chymotrypsin Substrate II, Fluorogenic, Suc-AAPF-AMC
Empirical Formula (Hill Notation):
C34H39N5O9
CAS Number:
88467-45-2
Molecular Weight:
661.70
MDL number:
MFCD00080246

Quality Level

100

Assay

≥98% (HPLC)

form

lyophilized solid

manufacturer/tradename

Calbiochem®

storage condition

OK to freeze
desiccated
protect from light

color

white

solubility

DMSO: soluble
acetonitrile: water (1:1): soluble

fluorescence

λex ~380 nm
λem ~460 nm

shipped in

ambient

storage temp.

−20°C

InChI

1S/C34H39N5O9/c1-19-16-30(43)48-27-18-23(11-12-24(19)27)37-25(17-22-8-5-4-6-9-22)32(45)38-33(46)26-10-7-15-39(26)34(47)21(3)36-31(44)20(2)35-28(40)13-14-29(41)42/h4-6,8-9,11-12,16,18,20-21,25-26,37H,7,10,13-15,17H2,1-3H3,(H,35,40)(H,36,44)(H,41,42)(H,38,45,46)/t20-,21-,25-,26-/m0/s1

InChI key

IGKPQFYMWKQDQS-KCXKOMAXSA-N

General description

Sensitive fluorogenic substrate for the quantitative determination of chymotrypsin activity (excitation maximum: ~380 nm; emission maximum: ~460 nm).
Sensitive fluorogenic substrate for the quantitative determination of chymotrypsin activity.

Biochem/physiol Actions

Cell permeable: no
Primary Target
Chymotrypsin
Product does not compete with ATP.
Reversible: no

Warning

Toxicity: Standard Handling (A)

Sequence

Suc-Ala-Ala-Pro-Phe-AMC

Reconstitution

Following reconstitution aliquot and freeze (-20°C). Avoid freeze/thaw cycles of solutions. Stock solutions are stable for up to 3 months at -20°C.

Other Notes

Irvine, G.B., et al. 1990. Anal. Biochem.185, 304.
Graf, L., et al. 1987. Biochemistry26, 2616.
Oshima, G. 1984. J. Biochem.95, 1131.
Oshima, G. 1983. J. Biochem.94, 1615.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

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