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251505-M

DAF-2 DA

Name: DAF-2 DA
Brand: MM

A cell-permeable derivative of DAF-2 that is hydrolyzed to DAF-2 by intracellular esterases.

Synonym(s):

DAF-2 DA, 4,5-Diaminofluorescein Diacetate

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About This Item

Empirical Formula (Hill Notation):

C₂₄H₁₈N₂O₇

CAS Number:

205391-02-2

Molecular Weight:

446.41

UNSPSC Code:

12171500

NACRES:

NA.25

MDL number:

MFCD05865101

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Properties

Quality Level

100

assay

≥98% (HPLC)

form

solution

manufacturer/tradename

Calbiochem^®

storage condition

OK to freeze, protect from light

color

pale yellow

fluorescence

λ_ex ~495 nm, λ_em ~515 nm

storage temp.

−20°C

SMILES string

Nc1cc2c(cc1N)C(=O)OC32c4c(cc(cc4)OC(=O)C)Oc5c3ccc(c5)OC(=O)C

InChI

1S/C24H18N2O7/c1-11(27)30-13-3-5-16-21(7-13)32-22-8-14(31-12(2)28)4-6-17(22)24(16)18-10-20(26)19(25)9-15(18)23(29)33-24/h3-10H,25-26H2,1-2H3

InChI key

PTSUYDXEEKDBQU-UHFFFAOYSA-N

Description

General description

A cell-permeable derivative of DAF-2 (Cat. No. 251500 ) that is hydrolyzed to DAF-2 by intracellular esterases. Can be used in fluorescence microscopy to measure real-time changes in NO levels in vivo.

Biochem/physiol Actions

Primary Target
Used to measure real-time changes in NO levels

Physical form

A 5 mM (1 mg/450 µl) solution in DMSO.

Preparation Note

Following initial thaw, aliquot and freeze (-20°C).

Other Notes

Berkels, R., et al. 2000. Cell Calcium27, 281.
Kojima, H., et al. 1998. Anal. Chem. 70, 2446.
Kojima, H., et al. 1998. Chem. Pharm. Bull. 46, 373.
Nakatsubo, N., et al. 1998. FEBS Lett. 427, 263.

Legal Information

Manufactured by Daiichi Pure Chemicals Co., Ltd

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Toxicity: Irritant (B)

Storage Class

10 - Combustible liquids

wgk

WGK 2

flash_point_f

188.6 °F - closed cup - (Dimethylsulfoxide)

flash_point_c

87 °C - closed cup - (Dimethylsulfoxide)

Certificates of Analysis (COA)

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Global Trade Item Number

SKU	GTIN
251505-1MG-M	04055977199239
251505-1MG	04055977199239