251505-M
DAF-2 DA
A cell-permeable derivative of DAF-2 that is hydrolyzed to DAF-2 by intracellular esterases.
别名:
DAF-2 DA, 4,5-Diaminofluorescein Diacetate
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关于此项目
经验公式（希尔记法）:
C24H18N2O7
化学文摘社编号:
分子量:
446.41
UNSPSC Code:
12171500
NACRES:
NA.25
MDL number:
SMILES string
Nc1cc2c(cc1N)C(=O)OC32c4c(cc(cc4)OC(=O)C)Oc5c3ccc(c5)OC(=O)C
InChI
1S/C24H18N2O7/c1-11(27)30-13-3-5-16-21(7-13)32-22-8-14(31-12(2)28)4-6-17(22)24(16)18-10-20(26)19(25)9-15(18)23(29)33-24/h3-10H,25-26H2,1-2H3
InChI key
PTSUYDXEEKDBQU-UHFFFAOYSA-N
assay
≥98% (HPLC)
form
solution
manufacturer/tradename
Calbiochem®
storage condition
OK to freeze, protect from light
color
pale yellow
fluorescence
λex ~495 nm, λem ~515 nm
storage temp.
−20°C
Quality Level
General description
A cell-permeable derivative of DAF-2 (Cat. No. 251500 ) that is hydrolyzed to DAF-2 by intracellular esterases. Can be used in fluorescence microscopy to measure real-time changes in NO levels in vivo.
Biochem/physiol Actions
Primary Target
Used to measure real-time changes in NO levels
Used to measure real-time changes in NO levels
Physical form
A 5 mM (1 mg/450 µl) solution in DMSO.
Preparation Note
Following initial thaw, aliquot and freeze (-20°C).
Other Notes
Berkels, R., et al. 2000. Cell Calcium27, 281.
Kojima, H., et al. 1998. Anal. Chem. 70, 2446.
Kojima, H., et al. 1998. Chem. Pharm. Bull. 46, 373.
Nakatsubo, N., et al. 1998. FEBS Lett. 427, 263.
Kojima, H., et al. 1998. Anal. Chem. 70, 2446.
Kojima, H., et al. 1998. Chem. Pharm. Bull. 46, 373.
Nakatsubo, N., et al. 1998. FEBS Lett. 427, 263.
Legal Information
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
Manufactured by Daiichi Pure Chemicals Co., Ltd
Disclaimer
Toxicity: Irritant (B)
存储类别
10 - Combustible liquids
wgk
WGK 2
flash_point_f
188.6 °F - closed cup - (Dimethylsulfoxide)
flash_point_c
87 °C - closed cup - (Dimethylsulfoxide)
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