260913
DNase I, Bovine Pancreas
Native DNase I from bovine pancreas.
Synonym(s):
DNase I, Bovine Pancreas, Deoxyribonuclease I
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About This Item
CAS Number:
UNSPSC Code:
12352202
NACRES:
NA.54
MDL number:
Specific activity:
≥60,000 dornase units/mg dry wt
Biological source:
bovine pancreas
biological source
bovine pancreas
Quality Segment
description
Merck USA index - 14, 2906
form
lyophilized
specific activity
≥60,000 dornase units/mg dry wt
manufacturer/tradename
Calbiochem®
storage condition
OK to freeze, desiccated
solubility
sodium acetate buffer: soluble, water: soluble
shipped in
ambient
storage temp.
−20°C
General description
Native DNase I from bovine pancreas. An endonuclease that preferentially cleaves phosphodiester linkages adjacent to pyrimidine nucleotides to yield 5′-phosphate terminated polynucleotides with a free hydroxyl group at the 3′ position. Acts on single and double stranded DNA. Note: 1 MU = 1,000,000 Dornase units
Note: 32 Dornase units equal ~1 Kunitz unit; 1 MU = 1,000,000 Dornase units.
Preparation Note
Following reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 3 months at -20°C.
Other Notes
One Dornase unit is defined as the amount of enzyme that will cause a decrease of 1.0 relative viscosity unit in a solution of highly polymerized DNA from the initial relative viscosity of 4.0 in 10 min at 30°C.
Takeshita, H., et al. 2000. Biochem. Biophys. Res. Commun.269, 481.
Jones, S.J., et al. 1996. J. Mol. Biol.264, 1154.
Jones, S.J., et al. 1996. J. Mol. Biol.264, 1154.
Legal Information
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Toxicity: Standard Handling (A)
signalword
Danger
hcodes
pcodes
Hazard Classifications
Resp. Sens. 1
Storage Class
11 - Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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