262015
DNA Base Excision Repair Pathway Inhibitor
The DNA Base Excision Repair Pathway Inhibitor, also referenced under CAS 6960-45-8, controls the biological activity of APE1 (human apurinic/apyrimidinic endonuclease.
Synonym(s):
DNA Base Excision Repair Pathway Inhibitor, 7-Nitroindole-2-carboxylic acid, CRT0044876
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About This Item
Empirical Formula (Hill Notation):
C9H6N2O4
CAS Number:
Molecular Weight:
206.15
UNSPSC Code:
12352200
NACRES:
NA.54
MDL number:
Assay:
≥98% (HPLC)
Form:
solid
Quality level:
Storage condition:
OK to freeze, protect from light
Quality Level
assay
≥98% (HPLC)
form
solid
manufacturer/tradename
Calbiochem®
storage condition
OK to freeze, protect from light
color
tan
solubility
DMSO: 5 mg/mL
shipped in
ambient
storage temp.
2-8°C
SMILES string
[N+](=O)([O-])c1c2[nH]c(cc2ccc1)C(=O)[O-]
InChI
1S/C9H6N2O4/c12-9(13)6-4-5-2-1-3-7(11(14)15)8(5)10-6/h1-4,10H,(H,12,13)/p-1
InChI key
BIUCOFQROHIAEO-UHFFFAOYSA-M
General description
A cell-permeable, potent, specific, and nontoxic inhibitor of the DNA repair enzyme, APE1 (human apurinic/apyrimidinic endonuclease; IC50 = ~3 µM). Shown to target the APE1 active site and inhibit its 3′-phosphodiesterase and 3′-phosphatase activities. Exhibits minimal effects on endonuclease IV, BamH1 restriction endonuclease, or topoisomerase I even at concentration as high as 100 µM. Also shown to potentiate the cytotoxicity of several DNA damaging agents, such as MMS, temozolomide, Zeocin, and H2O2 in HeLa, HT1080, and MDA-MB-231 cells.
Biochem/physiol Actions
Cell permeable: yes
Primary Target
APE 1
APE 1
Product does not compete with ATP.
Reversible: no
Target IC50: ~3 µM against APE1 (human apurinic/apyrimidinic endonuclease
Packaging
Packaged under inert gas
Preparation Note
Following reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 3 months at -20°C.
Other Notes
Madhusudan, S., et al. 2005. Nucl. Acids Res.33, 4711.
Legal Information
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Toxicity: Irritant (B)
wgk
WGK 3
signalword
Danger
hcodes
Hazard Classifications
Eye Irrit. 2 - Resp. Sens. 1
Storage Class
11 - Combustible Solids
flash_point_f
Not applicable
flash_point_c
Not applicable
Certificates of Analysis (COA)
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Global Trade Item Number
| SKU | GTIN |
|---|---|
| 262015-25MG | 04055977216974 |