287810
2′,7′-Dichlorofluorescin Diacetate
Cell-permeable fluorogenic probe that is useful for the detection of reactive oxygen species (ROS) and nitric oxide (•NO) and for the determination of the degree of overall oxidative stress.
Synonym(s):
2′,7′-Dichlorofluorescin Diacetate, H2DCFDA, 2ʹ,7ʹ-Dichlorodihydrofluorescein Diacetate
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About This Item
Empirical Formula (Hill Notation):
C24H16Cl2O7
CAS Number:
Molecular Weight:
487.29
UNSPSC Code:
12352202
NACRES:
NA.25
MDL number:
Assay:
≥94% (HPLC)
Form:
crystalline solid
Quality level:
Storage condition:
OK to freeze, protect from light
Quality Level
assay
≥94% (HPLC)
form
crystalline solid
manufacturer/tradename
Calbiochem®
storage condition
OK to freeze, protect from light
solubility
DMSO: 5 mg/mL
shipped in
ambient
storage temp.
−20°C
SMILES string
Clc1cc2c(cc1OC(=O)C)Oc3c(cc(c(c3)OC(=O)C)Cl)C2c4c(cccc4)C(=O)O
InChI
1S/C24H16Cl2O7/c1-11(27)31-21-9-19-15(7-17(21)25)23(13-5-3-4-6-14(13)24(29)30)16-8-18(26)22(32-12(2)28)10-20(16)33-19/h3-10,23H,1-2H3,(H,29,30)
InChI key
PXEZTIWVRVSYOK-UHFFFAOYSA-N
General description
Cell-permeable fluorogenic probe that is useful for the detection of reactive oxygen species (ROS) and the nitric oxide (NO) in neutrophils and macrophages and for the determination of the overall oxidative stress in toxicological phenomena.
Biochem/physiol Actions
Cell permeable: yes
Primary Target
Detection of reactive oxygen species (ROS) and nitric oxide (•NO)
Detection of reactive oxygen species (ROS) and nitric oxide (•NO)
Product does not compete with ATP.
Reversible: no
Packaging
Packaged under inert gas
Preparation Note
Following reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 3 months at -20°C.
Other Notes
Crow, J.P. 1997. Nitric Oxide 1, 145.
Ripple, M.O., et al. 1997. J. Natl. Cancer Inst. 89, 40.
Caro, A., and Puntarulo, S. 1996. Biochim. Biophys. Acta1291, 245.
Perez-Garcia, F., et al. 1996. Life Sci. 59, 2033.
Ramakrishnan, N., et al. 1996. Biochem. Pharmacol. 51, 1443.
Ferrer, A.S., et al. 1990. Anal. Biochem. 187, 129.
Ripple, M.O., et al. 1997. J. Natl. Cancer Inst. 89, 40.
Caro, A., and Puntarulo, S. 1996. Biochim. Biophys. Acta1291, 245.
Perez-Garcia, F., et al. 1996. Life Sci. 59, 2033.
Ramakrishnan, N., et al. 1996. Biochem. Pharmacol. 51, 1443.
Ferrer, A.S., et al. 1990. Anal. Biochem. 187, 129.
Legal Information
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Toxicity: Standard Handling (A)
Storage Class
11 - Combustible Solids
wgk
WGK 3
Certificates of Analysis (COA)
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Thivanka Muthumalage et al.
Toxics, 8(3) (2020-07-02)
Recently, there has been an outbreak associated with the use of e-cigarette or vaping products, associated lung injury (EVALI). The primary components of vaping products, vitamin E acetate (VEA) and medium-chain triglycerides (MCT), may be responsible for acute lung toxicity.
Hong-Wei Jiang et al.
Frontiers in physiology, 12, 662666-662666 (2021-07-16)
The aim of this study was to explore the protective effects and the regulatory mechanisms of bariatric surgery on kidney injury in diabetic rats. We established a useful type 2 diabetic rat model using high-fat and high-sugar diet feeding following
Shaiesh Yogeswaran et al.
Toxics, 9(10) (2021-10-23)
Studies have shown that aerosols generated from flavored e-cigarettes contain Reactive Oxygen Species (ROS), promoting oxidative stress-induced damage within pulmonary cells. Our lab investigated the ROS content of e-cigarette vapor generated from disposable flavored e-cigarettes (vape bars) with and without
Global Trade Item Number
| SKU | GTIN |
|---|---|
| 287810-100MG | 07790788054298 |