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Merck
CN

317210

DM-NITROPHEN Reagent, Tetrasodium Salt

Caged Ca2+ chelator that undergoes a major and rapid decrease in Ca2+-binding affinity upon photolysis.

Synonym(s):

DM-NITROPHEN Reagent, Tetrasodium Salt, 1-(2-Nitro-4,5-dimethoxyphenyl)-1,2-diaminoethane-N,N,Nʹ,Nʹ-tetraacetic Acid, 4Na, Dimethoxynitrophenamine, 4Na

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About This Item

Empirical Formula (Hill Notation):
C18H19N3O12 · 4Na
Molecular Weight:
561.32
NACRES:
NA.25
UNSPSC Code:
12352200

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assay

≥93% (HPLC)

form

solid

reaction suitability

reagent type: chelator

manufacturer/tradename

Calbiochem®

storage condition

OK to freeze, desiccated (hygroscopic), protect from light

color

orange

solubility

water: 100 mg/mL

shipped in

wet ice

storage temp.

−20°C

Quality Level

100

Related Categories

General description

Caged Ca2+ chelator that undergoes a major and rapid decrease in Ca2+-binding affinity upon photolysis. Can be microinjected or used with permeabilized cells. May also be useful as caged Mg2+ chelator. Kd (Ca2+) = 5 nM; Kd (Mg2+) = 2-5 µM.

Preparation Note

Following reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 3 months at -20°C.

Other Notes

Mulkey, R.M., and Zucker, R.S. 1993. J. Physiol. 462, 243.
Zucker, R.S. 1993. Cell Calcium 14, 87.
McCray, J.A., et al. 1992. Biochemistry31, 8856.
Delaney, K.R., and Zucker, R.S. 1990. J. Physiol.426, 473.
Kaplan, J.H., and Ellis-Davies, G.C. 1988. Proc. Natl. Acad. Sci. USA85, 6571.
Morad, M., et al. 1988. Science241, 842.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Toxicity: Standard Handling (A)

Storage Class

11 - Combustible Solids

wgk

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable

Certificates of Analysis (COA)

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