Skip to Content
Merck
CN

324405

Anti-Dopamine D4 Receptor Rabbit pAb

lyophilized, Calbiochem®

Sign In to View Organizational & Contract Pricing.

Select a Size

About This Item

NACRES:
NA.43
UNSPSC Code:
12352203

Key Documents

View All Documentation
Technical Service
Need help? Our team of experienced scientists is here for you.
Let Us Assist
Technical Service
Need help? Our team of experienced scientists is here for you.
Let Us Assist

Product Name

Anti-Dopamine D4 Receptor Rabbit pAb, lyophilized, Calbiochem®

biological source

rabbit

antibody form

serum

antibody product type

primary antibodies

clone

polyclonal

form

lyophilized

does not contain

preservative

species reactivity

human, rat

manufacturer/tradename

Calbiochem®

storage condition

OK to freeze

dilution

(ELISA
Immunoblotting (1:500)
Immunohistochemistry (1:10,000))

isotype

IgG

shipped in

ambient

storage temp.

2-8°C

target post-translational modification

unmodified

Quality Level

100

Gene Information

human ... DRD4(1815)
rat ... Drd4(25432)

General description

Recognizes the ~48-51 kDa dopamine D4 receptor in rat brain. Does not cross-react with other dopamine receptors.
Anti-Dopamine D4 Receptor, rabbit polyclonal, recognizes ~48-51 kDa dopamine D4 receptor in rat brain. Does not cross-react with other dopamine receptors. It is validated for use in ELISA, WB & IHC.
Rabbit polyclonal antibody supplied as lyophilized, undiluted serum. Recognizes the ~48-51 kDa D4 dopamine receptor.

Immunogen

Human
a synthetic peptide corresponding to amino acids 176-185 of human dopamine D₄ receptor

Other Notes

Mignini, F., et al. 2000. J. Autonom. Pharmacol.20, 325.
This antibody has also been reported to work for ELISA. For blocking immunoblot membranes, use ONLY 4% goat serum or evaporated goat′s milk. For immunohistochemistry, a 1:10,000 dilution of the reconstituted serum demonstrated specific staining of rat brain sections including olfactory bulb glomeruli, cortex layer 4, cortex layers 2 and 3 (dendrite staining), cortex layer 5 (apical dendrite staining), and the dentate gyrus of the hippocampus (diffuse staining in the molecular layer, punctuate staining in the granule cell layer).

Analysis Note

Positive Control
Rat brain

Application

ELISA (see comments)

Immunoblotting (1:500)

Immunohistochemistry (1:10,000)

Disclaimer

Toxicity: Standard Handling (A)

Physical form

Undiluted serum.

Preparation Note

Reconstitute the lyophilized antibody with 100 µl PBS containing 10 mg/ml BSA. For further dilution of the antiserum use a similar buffer formulation. Following reconstitution, refrigerate (4°C). Variables associated with assay conditions will dictate the proper working dilution.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Not finding the right product?  

Try our Product Selector Tool.

Storage Class

11 - Combustible Solids

wgk

WGK 2

flash_point_f

Not applicable

flash_point_c

Not applicable

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

Already Own This Product?

Find documentation for the products that you have recently purchased in the Document Library.

Visit the Document Library

Alberto Ricci et al.
The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society, 50(8), 1091-1096 (2002-07-23)
Dopamine D4 receptors mediate inhibition of vasopressin-dependent sodium reabsorption by dopamine in collecting tubules. At present, the distribution of D4 receptors in other renal districts remains an open issue. The renal distribution of D4 receptor was assessed in normally innervated
Satoko Oda et al.
Frontiers in neuroanatomy, 17, 1111008-1111008 (2023-03-04)
Pial astrocytes, a cellular component of the cerebral cortex surface structure, are observed in a wide range of mammalian species. Despite being recognized as such, the functional potential of pial astrocytes has long been overlooked. Our previous research demonstrated that
Dana C Borcherding et al.
PloS one, 6(9), e25537-e25537 (2011-10-04)
Dopamine (DA) binds to five receptors (DAR), classified by their ability to increase (D1R-like) or decrease (D2R-like) cAMP. In humans, most DA circulates as dopamine sulfate (DA-S), which can be de-conjugated to bioactive DA by arylsulfatase A (ARSA). The objective

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service