324676
Elastase, Pseudomonas aeruginosa
Elastase, Pseudomonas aeruginosa, is a native metallopeptidase that hydrolyzes insoluble elastin. Shares a significant sequence homology with thermolysin.
Synonym(s):
Pseudolysin
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Quality Segment
assay
≥90% (SDS-PAGE)
form
lyophilized
specific activity
≥250 units/mg protein
manufacturer/tradename
Calbiochem®
storage condition
OK to freeze
solubility
aqueous buffer: 1 mg/mL
shipped in
ambient
storage temp.
−20°C
General description
Native elastase from Pseudomonas aeruginosa. A metallopeptidase that hydrolyzes insoluble elastin. Shares a significant sequence homology with thermolysin. Pseudomonas aeruginosa elastase has been implicated in the reinforcement of the inflammation process in cases of infection with the bacterium.
Preparation Note
Following reconstitution, aliquot and freeze (-20°C) for long-term storage or refrigerate (4°C) for short-term storage. Stock solutions are stable for up to 1 week at 4°C or for up to 2 months at -20°C.
Other Notes
Azghani, A.O., et al. 2000. Lung178, 181.
Kon, Y., et al. 1999. FEMS Immunol. Med. Microbiol. 25, 313.
Braun, P., et al. 1998. J. Bacteriol. 180, 3467.
Kon, Y., et al. 1999. FEMS Immunol. Med. Microbiol. 25, 313.
Braun, P., et al. 1998. J. Bacteriol. 180, 3467.
One unit is defined as the amount of enzyme that will hydrolyze 1 µg insoluble elastin per h at 37°C.
Legal Information
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Toxicity: Standard Handling (A)
Storage Class
11 - Combustible Solids
WGK
WGK 1
Flash Point (°F)
Not applicable
Flash Point (°C)
Not applicable
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