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Merck
CN

324696

Millipore

Methoxysuccinyl-Ala-Ala-Pro-Val-p-nitroanilide

A specific chromogenic substrate for human neutrophil elastase.

Synonym(s):

Elastase Substrate I, Colorimetric

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About This Item

Empirical Formula (Hill Notation):
C27H38N6O9
CAS Number:
70967-90-7
Molecular Weight:
590.63
MDL number:
MFCD00077137
UNSPSC Code:
12352200
NACRES:
NA.54

Key Documents

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Product Name

Elastase Substrate I, Colorimetric, A specific chromogenic substrate for human neutrophil elastase.

Quality Level

100

Assay

≥95% (HPLC)

form

lyophilized

manufacturer/tradename

Calbiochem®

storage condition

OK to freeze
desiccated

solubility

water: 1 mg/mL

shipped in

ambient

storage temp.

−20°C

SMILES string

[N+](=O)([O-])c1ccc(cc1)N[C@@H](C(C)C)C(=O)NC(=O)[C@H]2N(CCC2)C(=O)[C@@H](NC(=O)[C@@H](NC(=O)CCC(=O)OC)C)C

InChI

1S/C27H38N6O9/c1-15(2)23(30-18-8-10-19(11-9-18)33(40)41)26(38)31-25(37)20-7-6-14-32(20)27(39)17(4)29-24(36)16(3)28-21(34)12-13-22(35)42-5/h8-11,15-17,20,23,30H,6-7,12-14H2,1-5H3,(H,28,34)(H,29,36)(H,31,37,38)/t16-,17-,20-,23-/m0/s1

InChI key

JWYKDZIEIWGSBN-OSAZLGQLSA-N

Related Categories

General description

A specific chromogenic substrate for human neutrophil elastase (Kcat/Km = 120,000 vs. 2700 for pancreatic elastase). Cleavage can be monitored at ~410 nm. Sold on the basis of substrate content.
Specific chromogenic substrate for human neutrophil elastase (kcat/Km = 120,000 versus pancreatic elastase: kcat/Km = 2,700). Cleavage can be monitored at 410 nm using ε = 8,800.

Biochem/physiol Actions

Cell permeable: no
Kcat/Km = 120,000 vs. 2700 for pancreatic elastase
Primary Target
Chromogenic substrate for human neutrophil elastase
Product does not compete with ATP.
Reversible: no

Physical form

Lyophilized from a solution containing 90 mg D-mannitol and 10 mg substrate.

Preparation Note

Following reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 3 months at -20°C.

Other Notes

Barrett, A.J., et al. 1989. Biochim. J.260, 259.
Nakajima, K., et al. 1979. J. Biol. Chem.254, 4027.
MeOSuc-Ala-Ala-Pro-Val-pNA

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Toxicity: Standard Handling (A)

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Ning Luan et al.
Toxins, 11(12) (2019-12-11)
Elastase is a globular glycoprotein and belongs to the chymotrypsin family. It is involved in several inflammatory cascades on the basis of cleaving the important connective tissue protein elastin, and is strictly regulated to a balance by several endogenous inhibitors.

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