Key Documents

View All Documentation

324713

Glu-C Protease

Name: Glu-C Protease
Brand: MM

Selectively cleaves at glutamic acid residues, suitable for mass spectromety, from Staphylococcus aureus

Synonym(s):

Endoproteinase Glu-C, Excision Grade, Staphylococcus aureus, V8 Protease

Select a Size

Change View

About This Item

CAS Number:

66676-43-5

EC Number:

3.4.21.19 (BRENDA, IUBMB)

UNSPSC Code:

12352202

NACRES:

NA.54

MDL number:

MFCD01324998

eCl@ss:

32160410

Specific activity:

≥10 units/mg protein

Biological source:

Staphylococcus aureus

Technical Service

Need help? Our team of experienced scientists is here for you.

Let Us Assist

Product Name

Endoproteinase Glu-C, Excision Grade, Staphylococcus aureus,

biological source

Staphylococcus aureus

Quality Level

100

grade

Proteomics Grade

form

lyophilized

specific activity

≥10 units/mg protein

manufacturer/tradename

Calbiochem^®

storage condition

OK to freeze

shipped in

ambient

storage temp.

2-8°C

Looking for similar products? Visit Product Comparison Guide

Related Categories

Proteases

Protein Mass Spectrometry

Proteins & Enzymes

General description

Native endoproteinase Glu-C from Staphylococcus aureus. Serine protease that specifically hydrolyzes peptide bonds at the carboxylic side of glutamic acid and aspartic acid residues. Inhibited by DFP and α₂-macroglobulin. Suggested working concentration: 1:20 to 1:100 (protease:protein by weight) for sequence analysis.

Preparation Note

Following reconstitution, aliquot and freeze (-20°C) for long term storage or refrigerate (4°C) for short term storage. Stock solutions are stable for up to 2 days at 4°C or for up to 1 month at -20°C.

Other Notes

Houmard, J., and Drapeau, G.R. 1972. Proc. Natl. Acad. Sci. USA69, 3506.

One unit is defined as the amount of enzyme that will hydrolyze 1.0 µmol Z-Phe-Leu-Glu-pNA per min at 25°C, pH 7.8.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Toxicity: Harmful (C)

pictograms

GHS08

signalword

Danger

hcodes

H317,H334

pcodes

P261 - P272 - P280 - P284 - P302 + P352 - P333 + P313

Hazard Classifications

Resp. Sens. 1 - Skin Sens. 1

Storage Class

11 - Combustible Solids

wgk

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

Already Own This Product?

Find documentation for the products that you have recently purchased in the Document Library.

Visit the Document Library

Selective activation of effector pathways by brain-specific G protein beta5.

S Zhang et al.

The Journal of biological chemistry, 271(52), 33575-33579 (1996-12-27)

While multiple G protein beta and gamma subunit isoforms have been identified, the implications of this potential diversity of betagamma heterodimers for signaling through betagamma-regulated effector pathways remains unclear. Furthermore the molecular mechanism(s) by which the betagamma complex modulates diverse

Citrullination of fibronectin alters integrin clustering and focal adhesion stability promoting stromal cell invasion.

Victoria L Stefanelli et al.

Matrix biology : journal of the International Society for Matrix Biology, 82, 86-104 (2019-04-21)

The extracellular matrix (ECM) microenvironment is increasingly implicated in the instruction of pathologically relevant cell behaviors, from aberrant transdifferentation to invasion and beyond. Indeed, pathologic ECMs possess a panoply of alterations that provide deleterious instructions to resident cells. Here we

Global Trade Item Number

SKU	GTIN
324713-50UG	07790788049171

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service