Key Documents

View All Documentation

324865

PhosphoDetect Anti-EGFR (pTyr⁸⁴⁵) Rabbit pAb

Name: PhosphoDetect Anti-EGFR (pTyr⁸⁴⁵) Rabbit pAb
Brand: MM

liquid, Calbiochem^®

Synonym(s):

Anti-Epidermal Growth Factor Receptor (pTyr⁸⁴⁵)

Select a Size

Change View

About This Item

NACRES:

NA.43

UNSPSC Code:

12352203

Clone:

polyclonal

Species reactivity:

human

Application:

—

Citations:

Technical Service

Need help? Our team of experienced scientists is here for you.

Let Us Assist

Properties

biological source

rabbit

Quality Level

100

antibody product type

primary antibodies

clone

polyclonal

form

liquid

contains

≤0.1% sodium azide as preservative

species reactivity

human

manufacturer/tradename

Calbiochem^®

storage condition

OK to freeze, avoid repeated freeze/thaw cycles

dilution

(Immunoblotting (1:1000))

isotype

IgG

shipped in

wet ice

storage temp.

−70°C

target post-translational modification

phosphorylation (pTyr845)

Gene Information

human ... EGFR(1956)

Description

General description

Rabbit polyclonal antibody adsorbed against non-phosphopeptide corresponding to the immunogen phosphorylation site and a generic tyrosine phosphorylated peptide, followed by immunoaffinity chromatography. Recognizes the ~185 KDa EGFR protein phosphorylated on Tyr⁸⁴⁵.

Recognizes the ~170 kDa EGFR protein phosphorylated at Tyr⁸⁴⁵ in EGF-treated A431 cells. Note: 1 T = 1 test. Sufficient for 10 Western miniblots.

This PhosphoDetect Anti-EGFR (pTyr⁸⁴⁵) Rabbit pAb is validated for use in Immunoblotting for the detection of EGFR (pTyr⁸⁴⁵).

Immunogen

a synthetic phosphopeptide corresponding to amino acids surrounding the Tyr⁸⁴⁵ phosphorylation site of human EGFR

Application

Immunoblotting (1:1000)

Physical form

In Dulbecco′s PBS (w/o Mg²⁺ and Ca²⁺), 1 mg/ml BSA, 50% glycerol, pH 7.4.

Preparation Note

Following initial thaw, aliquot and freeze (-70°C).

Analysis Note

Positive Control
A431 cells stimulated with EGF

Other Notes

Sieg, D.J. et al. 2000. Nat. Cell. Biol.2, 249.
Barbier, A.J. et al. 1999. J. Biol. Chem.274, 14067.
Biscardi, J.S., et al. 1999. J. Biol. Chem.274, 8335.
Hashimoto, A. et al. 1999. J. Biol. Chem.274, 20139
Poppleton, H.M. et al. 1999. Arch. Biochem. Biophys.363, 227.

The immunogen sequence is 100% conserved in mouse and rat and 93% conserved in chicken EGFR, but cross-reactivity has not been tested. When diluted to 1:1000 there is sufficient antibody to perform 10 miniblots (10 T) at 10 ml per blot. Variables associated with assay conditions will dictate the proper working dilution.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Toxicity: Standard Handling (A)

Still not finding the right product?

Explore all of our products under PhosphoDetect Anti-EGFR (pTyr⁸⁴⁵) Rabbit pAb

— or —

Try our Product Selector Tool to narrow your options

Storage Class

10 - Combustible liquids

wgk

WGK 1

flash_point_f

Not applicable

flash_point_c