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324867

Sigma-Aldrich

PhosphoDetect Anti-EGFR (pTyr¹⁰⁶⁸) Rabbit pAb

liquid, Calbiochem®

Synonym(s):

Anti-Epidermal Growth Factor Receptor (pTyr¹⁰⁶⁸)

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About This Item

UNSPSC Code:
12352203
NACRES:
NA.41

biological source

rabbit

Quality Level

100

antibody product type

primary antibodies

clone

polyclonal

form

liquid

contains

≤0.1% sodium azide as preservative

species reactivity

human, mouse, chicken, rat

manufacturer/tradename

Calbiochem®

storage condition

OK to freeze
avoid repeated freeze/thaw cycles

dilution

(Immunoblotting (1:1000))

isotype

IgG

shipped in

wet ice

storage temp.

−20°C

target post-translational modification

phosphorylation (pTyr1068)

Gene Information

chicken ... Egfr(396494)
human ... EGFR(1956)
mouse ... Egfr(13649)
rat ... Egfr(24329)

General description

Rabbit polyclonal antibody adsorbed against non-phosphorylation site, followed by immunoaffinity chromatography. Recognizes the ~170 kDa EGFR protein phosphorylated at Tyr1068.
Recognizes the ~185 kDa EGFR protein phosphorylated at Tyr1068. Note: 1 T = 1 test. Sufficient for 10 Western miniblots.
This PhosphoDetect Anti-EGFR (pTyr¹⁰⁶⁸) Rabbit pAb is validated for use in Immunoblotting for the detection of EGFR (pTyr¹⁰⁶⁸).

Immunogen

a synthetic phosphopeptide corresponding to amino acids surrounding the Tyr¹⁰⁶⁸ phosphorylation site of human EGFR

Application

Immunoblotting (1:1000)

Warning

Toxicity: Standard Handling (A)

Physical form

In Dulbecco′s PBS, 1 mg/ml BSA, 50% glycerol, pH 7.4.

Other Notes

Jo, M., et al. 2000. J. Biol. Chem.275, 8806.
Maudsley, S., et al. 2000. J. Biol. Chem.275, 9572.
Sieg, D.J. et al. 2000. Nat. Cell. Biol.2, 249.
Barbier, A.J. et al. 1999. J. Biol. Chem.274, 14067.
Hashimoto, A. et al. 1999. J. Biol. Chem.274, 20139
Poppleton, H.M. et al. 1999. Arch. Biochem. Biophys.363, 227.
Batzer, A.G. et al. 1994. Mol. Cell. Biol.14, 5192.
When diluted 1:1000 there is sufficient antibody to perform 10 miniblots (10 T) at 10 ml per blot. Variables associated with assay conditions will dictate the proper working dilution.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

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Storage Class Code

10 - Combustible liquids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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